PXD003738

PXD003738 is an original dataset announced via ProteomeXchange.

Title	Proteome and phophoproteome analysis of distinct dendritic cell maturation states
Description	Depending on their environment and their exposure to various factors, dendritic cells (DCs) exist in different activation and maturation states. The versatility of DCs allows them to shape the immune system towards an inflammatory or tolerant state depending on the antigens and the environment they encounter. In this study we aimed to provide a proteomic catalogue of differentially expressed and differentially phosphorylated proteins between distinct DC maturation states, brought about by bacteria that differ in their endotoxicity. To achieve this, we have performed unlabeled shotgun proteomics and phosphoproteomics on cell fractions obtained from murine bone marrow DC cultures. The symbiont B. vulgatus stimulation was used to obtain semi-mature DCs, and the pathobiont E. coli stimulation was used to obtain mature DCs. For both shotgun proteome and phosphoproteome analysis two biological replicates consisting of 8 mice were used. Each biological replicate were run as 3 technical replicates.
HostingRepository	PRIDE
AnnounceDate	2017-11-27
AnnouncementXML	Submission_2017-11-27_02:40:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marius Codrea
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-04 00:35:57	ID requested
⏵ 1	2017-11-27 02:40:14	announced

Korkmaz AG, Popov T, Peisl L, Codrea MC, Nahnsen S, Steimle A, Velic A, Macek B, von Bergen M, Bernhardt J, Frick JS, Proteome and phosphoproteome analysis of commensally induced dendritic cell maturation states. J Proteomics, 180():11-24(2018) [pubmed]

curator keyword: Biological

submitter keyword: Dendritic cells, maturation, symbionts, pathobionts, inflammation, shotgun proteomics, phosphoproteomics, signaling pathways, Voronoi treemaps

Prof. Dr. med. Julia-Stefanie Frick
contact affiliation	Institut für Medizinische Mikrobiologie und Hygiene Universitätsklinikum Tübingen Elfriede-Aulhorn-Str. 6, 72076 Tübingen Germany
contact email	Julia-Stefanie.Frick@med.uni-tuebingen.de
lab head
Marius Codrea
contact affiliation	Quantitative Biology Center (QBiC). Tuebingen, Germany
contact email	marius-cosmin.codrea@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD003738

PRIDE project URI

• PRIDE
  1. PXD003738
     1. Label: PRIDE project
     2. Name: Proteome and phophoproteome analysis of distinct dendritic cell maturation states