PXD003726 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach |
Description | Production of milk is a key characteristic of mammals, but the features of lactation vary greatly between monotreme, marsupial and eutherian mammals. Marsupials have a short gestation followed by a long lactation period, and the milk constituents vary greatly across lactation to meet the changing needs of the developing young. Marsupials are born immunologically naïve and rely on their mother’s milk for immunological protection. The koala is an iconic Australian species and many are increasingly threatened by disease. Here we use a mammary transcriptome, two milk proteomes and the koala genome to comprehensively characterise the protein components of the milk of the koala, with a key focus on the immune constituents during early and late lactation. We have characterised the most abundant peptides present in milk, examined key differences between early and late lactation, and determined differences between the koala and other marsupial species. The most abundant proteins were well characterised mammalian and marsupial-specific milk proteins including β-lactoglobulin, lactotransferrin, caseins and early lactation protein. We have generated a list of 851 immune compounds identified in the mammary transcriptome and examined a range of immune proteins that were highly abundant in the milk proteomes. These include immunoglobulins, complement components and immune receptors. A host of antimicrobial peptides were identified including cathelicidins, lysozyme, WAP four-disulfide core domain protein 2, Mucin-1 and Peptidoglycan recognition protein. We discovered that the previously described marsupial milk protein Very Early Lactation Protein is an ortholog of the eutherian gene Glycam1 (PP3) and likely has an antimicrobial role in milk. We also identified highly abundant koala endogenous retrovirus sequences in the mammary and milk transcriptome and proteomes, identifying a potential route for retroviral transmission from mother to young. Identifying and characterising the immune components of milk is key to understanding how marsupial young are protected throughout lactation and the novel immune compounds identified may have applications in clinical research. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:14:13.000.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thiri Zaw |
SpeciesList | scientific name: Phascolarctos cinereus (Koala); NCBI TaxID: 38626; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-03-02 02:00:44 | ID requested | |
1 | 2018-10-26 02:15:20 | announced | |
⏵ 2 | 2024-10-22 04:14:16 | announced | 2024-10-22: Updated project metadata. |
Publication List
Morris KM, O'Meally D, Zaw T, Song X, Gillett A, Molloy MP, Polkinghorne A, Belov K, Characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach. Sci Rep, 6():35011(2016) [pubmed] |
10.1038/srep35011; |
Keyword List
curator keyword: Biological |
submitter keyword: marsupials,Koala milk, transcriptomic, proteomic, nanoLC |
Contact List
Katherine Belov |
contact affiliation | Faculty of Veterinary Science, University of Sydney, Camperdown, NSW, 2006, Australia |
contact email | kathy.belov@sydney.edu.au |
lab head | |
Thiri Zaw |
contact affiliation | APAF |
contact email | tzaw@proteome.org.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003726
- Label: PRIDE project
- Name: Characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach