PXD003726

PXD003726 is an original dataset announced via ProteomeXchange.

Title	Characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach
Description	Production of milk is a key characteristic of mammals, but the features of lactation vary greatly between monotreme, marsupial and eutherian mammals. Marsupials have a short gestation followed by a long lactation period, and the milk constituents vary greatly across lactation to meet the changing needs of the developing young. Marsupials are born immunologically naïve and rely on their mother’s milk for immunological protection. The koala is an iconic Australian species and many are increasingly threatened by disease. Here we use a mammary transcriptome, two milk proteomes and the koala genome to comprehensively characterise the protein components of the milk of the koala, with a key focus on the immune constituents during early and late lactation. We have characterised the most abundant peptides present in milk, examined key differences between early and late lactation, and determined differences between the koala and other marsupial species. The most abundant proteins were well characterised mammalian and marsupial-specific milk proteins including β-lactoglobulin, lactotransferrin, caseins and early lactation protein. We have generated a list of 851 immune compounds identified in the mammary transcriptome and examined a range of immune proteins that were highly abundant in the milk proteomes. These include immunoglobulins, complement components and immune receptors. A host of antimicrobial peptides were identified including cathelicidins, lysozyme, WAP four-disulfide core domain protein 2, Mucin-1 and Peptidoglycan recognition protein. We discovered that the previously described marsupial milk protein Very Early Lactation Protein is an ortholog of the eutherian gene Glycam1 (PP3) and likely has an antimicrobial role in milk. We also identified highly abundant koala endogenous retrovirus sequences in the mammary and milk transcriptome and proteomes, identifying a potential route for retroviral transmission from mother to young. Identifying and characterising the immune components of milk is key to understanding how marsupial young are protected throughout lactation and the novel immune compounds identified may have applications in clinical research.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:13.000.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiri Zaw
SpeciesList	scientific name: Phascolarctos cinereus (Koala); NCBI TaxID: 38626;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-02 02:00:44	ID requested
1	2018-10-26 02:15:20	announced
⏵ 2	2024-10-22 04:14:16	announced	2024-10-22: Updated project metadata.

Morris KM, O'Meally D, Zaw T, Song X, Gillett A, Molloy MP, Polkinghorne A, Belov K, Characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach. Sci Rep, 6():35011(2016) [pubmed]

10.1038/srep35011;

curator keyword: Biological

submitter keyword: marsupials,Koala milk, transcriptomic, proteomic, nanoLC

Katherine Belov
contact affiliation	Faculty of Veterinary Science, University of Sydney, Camperdown, NSW, 2006, Australia
contact email	kathy.belov@sydney.edu.au
lab head
Thiri Zaw
contact affiliation	APAF
contact email	tzaw@proteome.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003726

PRIDE project URI

• PRIDE
  1. PXD003726
     1. Label: PRIDE project
     2. Name: Characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach