PXD003709

PXD003709 is an original dataset announced via ProteomeXchange.

Title	Preferential phosphorylation on old histones during early mitosis in human cells
Description	How histone posttranslational modifications (PTMs) are inherited through cell cycle remains poorly understood. Canonical histones are made in the S phase of cell cycle. Combining mass spectrometry-based technologies and stable isotope labeling by amino acids in cell culture (SILAC), we interrogate the distributions of multiple major histone PTMs on old versus new histones in synchronized human cells. We show that histone PTMs can be grouped to three categories accordingly to their distributions. Most lysine mono-methylation and acetylation PTMs are either symmetrically distributed on old and new histones, or asymmetric on new histones. In contrast, most di- and tri-methylation PTMs are asymmetric on old histones, suggesting the inheritance of different PTMs is regulated distinctly. Intriguingly, old and new histones are distinguished in their phosphorylation status during early mitosis, in three different human cell types: Hela, 293T and human foreskin fibroblast (HFF) cells. The mitotic hallmark H3S10ph is predominantly associated with old H3 at early mitosis and become symmetric with the progression of mitosis. The same distribution pattern is found on other mitotic histone phosphorylation marks, including H3T3/T6ph, H3.1/2S28ph and H1.4S26ph, excluding S28/S31ph on the H3 variant H3.3. Although H3S10ph often associates with the neighboring K9 di- or tri-methylation, they are not required for the asymmetric distribution of S10ph on the same H3 tail. Inhibition of the kinase Aurora B does not change the distribution pattern despite significant reduction of H3S10ph levels. K9me2 abundance on the new H3 is significantly reduced after Aurora B inhibition, suggesting a crosstalk between H3S10ph and H3K9me2.
HostingRepository	PRIDE
AnnounceDate	2016-06-01
AnnouncementXML	Submission_2016-06-01_01:02:53.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003709
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zuo-Fei Yuan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; propanoylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-29 02:17:50	ID requested
⏵ 1	2016-06-01 01:02:54	announced

Lin S, Yuan ZF, Han Y, Marchione DM, Garcia BA, Preferential Phosphorylation on Old Histones during Early Mitosis in Human Cells. J Biol Chem, 291(29):15342-57(2016) [pubmed]

curator keyword: Biological

submitter keyword: histone modifications, histone phosphorylation, cell cycle, mitosis, mass spectrometry

Benjamin A. Garcia
contact affiliation	Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
contact email	bgarci@mail.med.upenn.edu
lab head
Zuo-Fei Yuan
contact affiliation	UPenn
contact email	zuoyuan@mail.med.upenn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003709
     1. Label: PRIDE project
     2. Name: Preferential phosphorylation on old histones during early mitosis in human cells