PXD003697

PXD003697 is an original dataset announced via ProteomeXchange.

Title	Gel based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire
Description	Plasma membrane (PM) encompasses total cellular contents, serves as a living barrier regulates all cellular exchanges in a spatiotemporal fashion. Most of the essential tasks of PMs including molecular transport, cell-cell interaction and signal transduction are carried out by their proteinaceous components, which make the PM protein repertoire to be diverse and dynamic. Here, we report the systematic analysis of PM proteome of a food legume, chickpea and have developed a PM proteome reference map. Proteins were extracted from highly enriched PM fractions using aqueous two-phase partitioning. To address a population of PM proteins as comprehensive as possible, both gel-based and gel-free approaches were employed, which led to the identification of a set of 2754 non-redundant proteins. These included both integral membrane proteins having bilayer spanning domains as well as peripheral proteins associated with PMs through protein-protein interactionsor post-translational modifications.
HostingRepository	PRIDE
AnnounceDate	2024-12-05
AnnouncementXML	Submission_2024-12-05_03:39:14.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niranjan Chakraborty
SpeciesList	scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-26 00:50:07	ID requested
⏵ 1	2024-12-05 03:39:15	announced

10.1016/j.jprot.2016.04.015;

Barua P, Subba P, Lande NV, Mangalaparthi KK, Prasad TSK, Chakraborty S, Chakraborty N, Gel-based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire. J Proteomics, 143():199-208(2016) [pubmed]

curator keyword: Biological

submitter keyword: shotgun proteomics,Plant proteomics, high resolution mass spectrometry

Dr. Niranjan Chakraborty
contact affiliation	Lab no. 104 National Institute of Plant Genome Research ArunaAsaf Ali Marg New Delhi- 110067
contact email	nchakraborty@nipgr.ac.in
lab head
Niranjan Chakraborty
contact affiliation	Scientist
contact email	nchakraborty@nipgr.ac.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD003697

PRIDE project URI

• PRIDE
  1. PXD003697
     1. Label: PRIDE project
     2. Name: Gel based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire