PXD003691

PXD003691 is an original dataset announced via ProteomeXchange.

Title	Human oocyte proteome and secretome
Description	The proteome and secretome of human oocytes was studied by shotgun LCMSMS, starting from pools of 100 cells. Exploiting SP3, a novel technology for proteomic sample preparation using magnetic beads, we scaled down proteome analysis to single cells. Despite the low protein content of only ~100ng per cell, we consistently identified ~450 proteins from individual oocytes. When comparing proteomes of individual oocytes at the germinal vesicle (GV) and metaphase II (MII) stage by label-free quantification, we found several proteins preferentially expressed in matuare and immature cells. This study demonstrates that an innovative proteomics workflow facilitates analysis of single human oocytes to investigate human oocyte biology and pre-implantation development. The approach presented here paves the way for quantitative proteomics in other quantity-limited tissues and cell types.
HostingRepository	PRIDE
AnnounceDate	2016-05-26
AnnouncementXML	Submission_2016-05-26_06:00:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeroen Krijgsveld
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-25 05:25:51	ID requested
⏵ 1	2016-05-26 06:00:21	announced

Virant-Klun I, Leicht S, Hughes C, Krijgsveld J, Identification of Maturation-Specific Proteins by Single-Cell Proteomics of Human Oocytes. Mol Cell Proteomics, 15(8):2616-27(2016) [pubmed]

curator keyword: Biological

submitter keyword: Human, oocytes, single-cell

Jeroen Krijgsveld
contact affiliation	German Cancer Research Center & Heidelberg University
contact email	j.krijgsveld@dkfz.de
lab head
Jeroen Krijgsveld
contact affiliation	DKFZ
contact email	j.krijgsveld@dkfz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD003691

PRIDE project URI

• PRIDE
  1. PXD003691
     1. Label: PRIDE project
     2. Name: Human oocyte proteome and secretome