PXD003684

PXD003684 is an original dataset announced via ProteomeXchange.

Title	Analysis of isolated Plastoglobules from Arabidopsis thaliana WT and M48 null mutants.
Description	We studied the function of PG localized protease, PGM48 both in vitro and in vivo during natural senescence in Arabidopsis thaliana.Our results show that PGM48 is a metalloprotease and a senescence inducible protein. In vitro assay by recombinant protein revealed a proteolytic activity. Quantitative proteome analysis of PGs from senescing rosettes of overexpression plants lines showed a significantly reduced level of CAROTENOID CLEAVAGE ENZYME 4 ( CCD4) protein but 2-3 fold higher level of phytyl-esterase (PES1) compared to wild type; the opposite results were found in RNAi lines. Yeast-2-hybrid analysis and in vitro pull-down experiments showed that PGM48 interacts with the kinase ABC1K3, PES1 and CCD4, but not with chlorophyll catabolic enzymes. We propose that PGM48 acts as a positive regulator of leaf senescence.
HostingRepository	PRIDE
AnnounceDate	2019-04-09
AnnouncementXML	Submission_2019-04-09_05:01:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003684
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Giulia Friso
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-25 01:21:12	ID requested
⏵ 1	2019-04-09 05:01:04	announced

Bhuiyan NH, Friso G, Rowland E, Majsec K, van Wijk KJ, The Plastoglobule-Localized Metallopeptidase PGM48 Is a Positive Regulator of Senescence in Arabidopsis thaliana. Plant Cell, 28(12):3020-3037(2016) [pubmed]

curator keyword: Biological

submitter keyword: Plastoglobules, protease, Arabidopsis thaliana, chloroplast, quantitative proteomics, LC-MSMS, LTQ-Orbitrap

Giulia Friso c/o Klaas van Wijk's Lab
contact affiliation	Cornell University, Ithaca, NY (USA)
contact email	gf32@cornell.edu
lab head
Giulia Friso
contact affiliation	Plant Biology
contact email	gf32@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD003684

PRIDE project URI

• PRIDE
  1. PXD003684
     1. Label: PRIDE project
     2. Name: Analysis of isolated Plastoglobules from Arabidopsis thaliana WT and M48 null mutants.