PXD003682

PXD003682 is an original dataset announced via ProteomeXchange.

Title	Systematic analysis of the lysine acetylome in Beauveria bassiana
Description	Lysine acetylation emerging as a ubiquitous and conserved posttranslational modification plays an important regulatory role in almost every aspect of eukaryotes and prokaryotes. To gain insight into the nature, extent and biological function of lysine acetylation in Beauveria bassiana, a filamentous entomopathogenic fungus, we used immunoaffinity-based acetyl-lysine peptide enrichment integrated with high resolution mass spectrometry to comprehensively characterize lysine acetylated proteins in this fungus. Here we identified a total of 283 proteins with 464 acetylated sites, representing the first acetylproteome reported to date in filamentous fungi. Bioinformatics analysis of this acetylome showed that the acetylated proteins are involved in a wide range of cellular functions, such as metabolism, transcription, and exhibit diverse subcellular localizations. Enrichment of molecular function, biological process, and KEGG pathway implied that identified acetylated proteins of B. bassiana were very important in chromatin organization, ribosome, nucleosome assembly, carbon metabolism, and biosynthesis of secondary metabolites. Moreover, we matched five conserved lysine acetylated motifs containing of KacY, KacH, KacF, FxKac, KacxxxxK and one specific motif KacW in B. bassiana. Taken together, our acetylome analysis revealed a surprising breadth of cellular processes affected by lysine acetylation and also furnishes some fresh intervention nodes for the rational improvement of the friednly entomopathogenic fungus.
HostingRepository	PRIDE
AnnounceDate	2017-11-02
AnnouncementXML	Submission_2017-11-02_04:44:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wang zhikang
SpeciesList	scientific name: Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus) (Tritirachium shiotae); NCBI TaxID: 655819;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-25 01:19:56	ID requested
⏵ 1	2017-11-02 04:44:35	announced

Wang ZK, Cai Q, Liu J, Ying SH, Feng MG, Global Insight into Lysine Acetylation Events and Their Links to Biological Aspects in Beauveria bassiana, a Fungal Insect Pathogen. Sci Rep, 7():44360(2017) [pubmed]

curator keyword: Biological

submitter keyword: Entomopathogenic fungi

lysine acetylation

acetylome

asexual development

stress responses

Zhi-Kang Wang
contact affiliation	Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
contact email	wangzhikang10@163.com
lab head
wang zhikang
contact affiliation	zhejiang university
contact email	wangzhikang10@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003682
     1. Label: PRIDE project
     2. Name: Systematic analysis of the lysine acetylome in Beauveria bassiana