PXD003678

PXD003678 is an original dataset announced via ProteomeXchange.

Title	Dam1 complex-microtubule interaction
Description	we used cross-linking mass spectrometry to determine the molecular architecture of the Dam1 complex alone and bound to microtubules. Our data provide a map of the subunit arrangement of the Dam1 complex. Analysis of the Dam1 complex assembled around microtubules reveals that the Spc34 and Ask1 subunits are likely involved in self -assembly whereas the Duo1 and Dam1 subunits both interact with microtubules. We also demonstrate that the C termini of Dam1 and Duo1 provide the microtubule binding properties to the Dam1 complex. Our data provides key information on the organization of the Dam1 complex around microtubules.
HostingRepository	PRIDE
AnnounceDate	2016-03-01
AnnouncementXML	Submission_2016-03-14_02:33:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Zou
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-23 02:22:10	ID requested
1	2016-03-01 01:43:28	announced
⏵ 2	2016-03-14 02:33:26	announced	Updated publication reference for PubMed record(s): 26962051.

Legal T, Zou J, Sochaj A, Rappsilber J, Welburn JP, Molecular architecture of the Dam1 complex-microtubule interaction. Open Biol, 6(3):(2016) [pubmed]

curator keyword: Biological

submitter keyword: microtubule, Dam1 complex, Mass Spectrometry, cross-linking

Prof. Juri Rappsilber
contact affiliation	Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh Michael Swann Building 4.18a, Max Born Crescent, Edinburgh EH9 3BF
contact email	juri.rappsilber@ed.ac.uk
lab head
Juan Zou
contact affiliation	The University of Edinburgh
contact email	jzou@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD003678

PRIDE project URI

• PRIDE
  1. PXD003678
     1. Label: PRIDE project
     2. Name: Dam1 complex-microtubule interaction