PXD003669

PXD003669 is an original dataset announced via ProteomeXchange.

Title	Identification of type strain-specific diagnostic peptides of the Bacillus cereus group
Description	The Bacillus cereus group consists of eight very closely related species and comprises both harmless and human pathogenic species. Yet, methods to rapidly and accurately distinguish these species are currently lacking as we demonstrate that classical matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) biotyping did not achieve reliable identification of each type strain. We assigned type strain-specific diagnostic peptides to the B. cereus group based on comparisons of their proteome profiles. The number of diagnostic peptides varies remarkably in type strain-dependent manner. The state of the art of the reference database is crucial in the process of validating candidate diagnostic peptides and may lead to a noteworthy reduction of verified diagnostic peptides as putative diagnostic peptides might be found in other species as well.
HostingRepository	PRIDE
AnnounceDate	2018-02-07
AnnouncementXML	Submission_2019-02-12_08:04:40.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003669
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Drissner
SpeciesList	scientific name: Bacillus weihenstephanensis NBRC 101238 = DSM 11821; NCBI TaxID: 1220585; scientific name: Bacillus cereus (strain ATCC 14579 / DSM 31); NCBI TaxID: 226900; scientific name: Bacillus mycoides DSM 2048; NCBI TaxID: 526997; scientific name: Bacillus thuringiensis serovar berliner ATCC 10792; NCBI TaxID: 527031; scientific name: Bacillus toyonensis BCT-7112; NCBI TaxID: 1415784; scientific name: Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98); NCBI TaxID: 315749; scientific name: Bacillus pseudomycoides DSM 12442; NCBI TaxID: 527000;
ModificationList	Deamidated; Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-22 00:27:58	ID requested
1	2018-02-07 06:33:14	announced
⏵ 2	2019-02-12 08:04:42	announced	Updated publication reference for PubMed record(s): 27432653.

Pfrunder S, Grossmann J, Hunziker P, Brunisholz R, Gekenidis MT, Drissner D, Bacillus cereus Group-Type Strain-Specific Diagnostic Peptides. J Proteome Res, 15(9):3098-107(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Bacillus cereus group, type strains, diagnostic peptides, LC-MS/MS

David Drissner
contact affiliation	Agroscope Institute for Food Sciences Schloss 1 8820 Waedenswil Switzerland
contact email	david.drissner@agroscope.admin.ch
lab head
David Drissner
contact affiliation	Agroscope, Institute for Food Sciences, 8820 Waedenswil, Switzerland
contact email	david.drissner@agroscope.admin.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003669
     1. Label: PRIDE project
     2. Name: Identification of type strain-specific diagnostic peptides of the Bacillus cereus group