PXD003664

PXD003664 is an original dataset announced via ProteomeXchange.

Title	Serial interactome capture of the human cell nucleus
Description	Novel RNA-guided cellular functions are paralleled by an increasing number of RNA binding proteins (RBPs). We present “serial interactome capture” (serIC), a multiple purification procedure of UV-crosslinked poly(A)-RNA-protein complexes that enables global RBP detection with maximal specificity. We apply serIC to nuclei of proliferating K562 cells to obtain the first human nuclear interactome. The domain composition of the 382 identified nuclear RBPs markedly differs from previous IC experiments, including fewer factors without known RNA binding domains that are in better agreement with computationally predicted RNA binding. serIC extends the number of DNA-RNA binding proteins (DRBPs), and reveals a network of RBPs involved in p53 signaling and double strand break repair. serIC is an effective tool to couple global RBP capture with additional selection or labelling steps for specific detection of highly purified RBPs. The nuclear interactome presented here is a stepping-stone towards deciphering of the functional RNA-protein network in the mammalian nucleus.
HostingRepository	PRIDE
AnnounceDate	2016-03-31
AnnouncementXML	Submission_2016-04-06_00:41:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-19 04:25:57	ID requested
1	2016-03-31 05:35:10	announced
⏵ 2	2016-04-06 00:41:23	announced	Updated publication reference for PubMed record(s): 27040163.

Conrad T, Albrecht AS, de Melo Costa VR, Sauer S, Meierhofer D, Ø, rom UA, Serial interactome capture of the human cell nucleus. Nat Commun, 7():11212(2016) [pubmed]

curator keyword: Biological

submitter keyword: RNA binding proteins

interactome capture

nuclear RNA binding proteome

Ulf Örom
contact affiliation	Max Planck Institute for Molecular Genetics, Otto Warburg Laboratories, 14195 Berlin, Germany
contact email	oerom@molgen.mpg.de
lab head
David Meierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD003664

PRIDE project URI

• PRIDE
  1. PXD003664
     1. Label: PRIDE project
     2. Name: Serial interactome capture of the human cell nucleus