PXD003652

PXD003652 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	S-acylation and fatty acylation in Naïve and LPS/IFN- gamma activated Raw264.7
Description	The advances in chemical proteomics have significantly expanded our understanding of the diversity and abundance of fatty-acylated proteins in eukaryotes, and reveal novel functions for these lipid protein modifications. Nonetheless, quantitative comparative proteomic analysis of fatty-acylated proteins in different cellular states is still challenging. To address these limitations, we systematically evaluated different proteomic methods (alk-16 chemical reporter and acyl-RAC) and established robust conditions to selectively and quantitatively profile fatty-acylated proteins in mammalian cells. Using a combination of metabolic labeling with fatty acid chemical reporters, selective chemical enrichment and label-free proteomics, we performed a quantitative analysis of fatty-acylated proteins in naïve and activated macrophages. These studies revealed novel fatty-acylated proteins associated with host immunity that are differently expressed and lipid-modified in different cellular states.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:19.875.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuelle Thinon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N-ethylmaleimide derivatized cysteine; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-17 01:48:49	ID requested
1	2018-03-27 23:55:11	announced
⏵ 2	2024-10-22 04:10:22	announced	2024-10-22: Updated project metadata.

Publication List

10.1021/acs.jproteome.8b00002;
Thinon E, Fernandez JP, Molina H, Hang HC, Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites. J Proteome Res, 17(5):1907-1922(2018) [pubmed]

10.1021/acs.jproteome.8b00002;

Thinon E, Fernandez JP, Molina H, Hang HC, Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites. J Proteome Res, 17(5):1907-1922(2018) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: RAW264.7, post translational modifications, S-acylation,fatty acylation, S-palmitoylation

curator keyword: Biological

submitter keyword: RAW264.7, post translational modifications, S-acylation,fatty acylation, S-palmitoylation

Contact List

Howard C Hang
contact affiliation	Laboratory of Chemical Biology and Microbial Pathogenesis The Rockefeller University New York NY, USA
contact email	hhang@rockefeller.edu
lab head
Emmanuelle Thinon
contact affiliation	The Francis Crick Institute
contact email	emmanuellethinon@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD003652
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD003652

PRIDE project URI

Repository Record List

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