PXD003650 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of soybean root including hypocotyl during recovery after drought stress |
Description | Soybean is a nutritionally important crop that exhibits reductions in growth and yields under drought stress. To investigate soybean responses during post-drought recovery, a gel-free proteomic technique was used to examine the protein profile. Two-day-old soybeans were stressed with drought for 4 days, recovered for 4 days, and root including hypocotyl was collected under drought and during the recovery stage. Morphological analysis revealed that growth was suppressed under drought stress that recovered following stress removal. Malondialdehyde content was increased under drought stress but returned to normal during the recovery stage. A total of 792 and 888 proteins were identified in control and drought-stressed root including hypocotyl during recovery stage, respectively. Based on the proteomic and cluster analyses, peroxidase and aldehyde dehydrogenase were significantly changed at analyzed time points under drought stress and during recovery. The activity of peroxidase was decreased under drought stress but increased during recovery. The activity of aldehyde dehydrogenase was increased under drought stress but returned to normal during the recovery stage. These results suggest that peroxidase and aldehyde dehydrogenase play key role in post-drought recovery in soybean by scavenging toxic reactive oxygen species and reducing harmful aldehydes load. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:10:28.193.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mudassar Khan |
SpeciesList | scientific name: Glycine max; NCBI TaxID: 3847; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-16 23:57:16 | ID requested | |
1 | 2016-06-16 06:39:18 | announced | |
⏵ 2 | 2024-10-22 04:10:31 | announced | 2024-10-22: Updated project metadata. |
Publication List
Khan MN, Komatsu S, Proteomic analysis of soybean root including hypocotyl during recovery from drought stress. J Proteomics, 144():39-50(2016) [pubmed] |
10.1016/j.jprot.2016.06.006; |
Keyword List
curator keyword: Biological |
submitter keyword: proteomics, recovery, root, soybean, drought |
Contact List
Setsuko KOMATSU |
contact affiliation | Chief Scientist, National Institute of Crop Science, Tsukuba, Japan |
contact email | komatsu.setsuko.fu@u.tsukuba.ac.jp |
lab head | |
Mudassar Khan |
contact affiliation | University of Tsukuba |
contact email | mnkhan@affrc.go.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003650
- Label: PRIDE project
- Name: Proteomic analysis of soybean root including hypocotyl during recovery after drought stress