PXD003650

PXD003650 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of soybean root including hypocotyl during recovery after drought stress
Description	Soybean is a nutritionally important crop that exhibits reductions in growth and yields under drought stress. To investigate soybean responses during post-drought recovery, a gel-free proteomic technique was used to examine the protein profile. Two-day-old soybeans were stressed with drought for 4 days, recovered for 4 days, and root including hypocotyl was collected under drought and during the recovery stage. Morphological analysis revealed that growth was suppressed under drought stress that recovered following stress removal. Malondialdehyde content was increased under drought stress but returned to normal during the recovery stage. A total of 792 and 888 proteins were identified in control and drought-stressed root including hypocotyl during recovery stage, respectively. Based on the proteomic and cluster analyses, peroxidase and aldehyde dehydrogenase were significantly changed at analyzed time points under drought stress and during recovery. The activity of peroxidase was decreased under drought stress but increased during recovery. The activity of aldehyde dehydrogenase was increased under drought stress but returned to normal during the recovery stage. These results suggest that peroxidase and aldehyde dehydrogenase play key role in post-drought recovery in soybean by scavenging toxic reactive oxygen species and reducing harmful aldehydes load.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:28.193.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mudassar Khan
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ XL

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-16 23:57:16	ID requested
1	2016-06-16 06:39:18	announced
⏵ 2	2024-10-22 04:10:31	announced	2024-10-22: Updated project metadata.

Khan MN, Komatsu S, Proteomic analysis of soybean root including hypocotyl during recovery from drought stress. J Proteomics, 144():39-50(2016) [pubmed]

10.1016/j.jprot.2016.06.006;

curator keyword: Biological

submitter keyword: proteomics, recovery, root, soybean, drought

Setsuko KOMATSU
contact affiliation	Chief Scientist, National Institute of Crop Science, Tsukuba, Japan
contact email	komatsu.setsuko.fu@u.tsukuba.ac.jp
lab head
Mudassar Khan
contact affiliation	University of Tsukuba
contact email	mnkhan@affrc.go.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003650
     1. Label: PRIDE project
     2. Name: Proteomic analysis of soybean root including hypocotyl during recovery after drought stress