PXD003644 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein profiling and identification of new biomarkers of brain ischemia by MALDI-imaging-mass-spectrometry |
Description | Introduction The identification of proteins involved in brain ischemia might allow the discovery of new putative biomarkers or potential therapeutic target of one of the most important neurological disorders. MALDI Imaging-Mass-Spectrometry (IMS) is a powerful technique that allows the visualization of protein distribution along a tissue without labeling. Our aim is to study the distribution of proteins along mouse brain after an ischemic insult and to identify relevant proteins involved in brain ischemia. Materials and methods We occluded the middle cerebral artery (60min) of C57BL/6J mice (n=4) with 24h of reperfusion. Brain slices were analyzed by MALDI-TOF and mass spectra from infarct (IC) and contralateral (CL) regions were compared using ClinProTools. Relevant m/z were selected after PCA analysis and their ion distribution maps were analyzed by FlexImagin3.0. Protein identification was conducted on mouse brain homogenates through a bottom-up approach consisting on complementary fractionations based on tricine gels and RP-HPLC. The identifications were confirmed by immunohistochemistry. Results We identified 102 m/z with different abundances between IC and CL (p<0.05), from which 21 m/z were selected by PCA as more relevant. Thirteen of them were found increased in the infarct region and 4 m/z showed a discrimination higher than 90% between IC and CL. After bottom-up identification, immunohistochemistry analysis confirmed increased ATP5i and COX6C expressions, and decreased UMP-CMP kinase in IC compared to CL. Conclusions We identified for the first time by MALDI-IMS several m/z peaks with different abundances between IC and CL. These proteins involved in brain ischemia might represent potential diagnostic biomarkers or target molecules for neurological recovery. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_02:56:42.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003644 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Víctor Llombart |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-16 02:19:49 | ID requested | |
1 | 2016-12-21 05:07:19 | announced | |
⏵ 2 | 2017-10-24 02:56:43 | announced | Updated project metadata. |
Publication List
Llombart V, Trejo SA, Bronsoms S, Morancho A, Feifei M, Faura J, Garc, í, a-Berrocoso T, Simats A, Rosell A, Canals F, Hern, á, ndez-Guillam, ó, n M, Montaner J, Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry. J Proteomics, 152():243-253(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: MALDI imaging, brain ischemia, tMCAO, biomarkers |
Contact List
Joan Montaner |
contact affiliation | vall hebron institute of research |
contact email | montaner.villalonga@gmail.com |
lab head | |
Víctor Llombart |
contact affiliation | Vall Hebrón Institute of Research |
contact email | llombs@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003644
- Label: PRIDE project
- Name: Protein profiling and identification of new biomarkers of brain ischemia by MALDI-imaging-mass-spectrometry