PXD003644

PXD003644 is an original dataset announced via ProteomeXchange.

Title	Protein profiling and identification of new biomarkers of brain ischemia by MALDI-imaging-mass-spectrometry
Description	Introduction The identification of proteins involved in brain ischemia might allow the discovery of new putative biomarkers or potential therapeutic target of one of the most important neurological disorders. MALDI Imaging-Mass-Spectrometry (IMS) is a powerful technique that allows the visualization of protein distribution along a tissue without labeling. Our aim is to study the distribution of proteins along mouse brain after an ischemic insult and to identify relevant proteins involved in brain ischemia. Materials and methods We occluded the middle cerebral artery (60min) of C57BL/6J mice (n=4) with 24h of reperfusion. Brain slices were analyzed by MALDI-TOF and mass spectra from infarct (IC) and contralateral (CL) regions were compared using ClinProTools. Relevant m/z were selected after PCA analysis and their ion distribution maps were analyzed by FlexImagin3.0. Protein identification was conducted on mouse brain homogenates through a bottom-up approach consisting on complementary fractionations based on tricine gels and RP-HPLC. The identifications were confirmed by immunohistochemistry. Results We identified 102 m/z with different abundances between IC and CL (p<0.05), from which 21 m/z were selected by PCA as more relevant. Thirteen of them were found increased in the infarct region and 4 m/z showed a discrimination higher than 90% between IC and CL. After bottom-up identification, immunohistochemistry analysis confirmed increased ATP5i and COX6C expressions, and decreased UMP-CMP kinase in IC compared to CL. Conclusions We identified for the first time by MALDI-IMS several m/z peaks with different abundances between IC and CL. These proteins involved in brain ischemia might represent potential diagnostic biomarkers or target molecules for neurological recovery.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_02:56:42.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003644
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Víctor Llombart
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-16 02:19:49	ID requested
1	2016-12-21 05:07:19	announced
⏵ 2	2017-10-24 02:56:43	announced	Updated project metadata.

Llombart V, Trejo SA, Bronsoms S, Morancho A, Feifei M, Faura J, Garc, í, a-Berrocoso T, Simats A, Rosell A, Canals F, Hern, á, ndez-Guillam, ó, n M, Montaner J, Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry. J Proteomics, 152():243-253(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: MALDI imaging, brain ischemia, tMCAO, biomarkers

Joan Montaner
contact affiliation	vall hebron institute of research
contact email	montaner.villalonga@gmail.com
lab head
Víctor Llombart
contact affiliation	Vall Hebrón Institute of Research
contact email	llombs@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003644
     1. Label: PRIDE project
     2. Name: Protein profiling and identification of new biomarkers of brain ischemia by MALDI-imaging-mass-spectrometry