PXD003640

PXD003640 is an original dataset announced via ProteomeXchange.

Title	ON THE ACCURACY, PRECISION AND STATISTICAL SIGNIFICANCE OF COMPRESSED RATIOS IN ISOBARIC LABELLING
Description	Despite the outstanding advantages that isobaric labelling has to offer in quantitative proteomics, its precision and accuracy are undermined by ratio compression, which is consequence of the co-isolation of peptides within a selected isolation window. Here, using the two-proteomes approach we investigated the relationships between precision and accuracy on the statistical significance of peptide and protein ratios in three types of mass spectrometers.
HostingRepository	PRIDE
AnnounceDate	2016-08-08
AnnouncementXML	Submission_2016-08-08_09:49:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Martinez
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics maXis series; LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-16 01:09:46	ID requested
⏵ 1	2016-08-08 09:49:30	announced

Martinez-Val A, Garcia F, Xim, é, nez-Emb, ú, n P, Ibarz N, Zarzuela E, Ruppen I, Mohammed S, Munoz J, On the Statistical Significance of Compressed Ratios in Isobaric Labeling: A Cross-Platform Comparison. J Proteome Res, 15(9):3029-38(2016) [pubmed]

curator keyword: Technical

submitter keyword: isobaric labelling, ratio compression, statistics, precision, accuracy

Javier Muñoz Peralta
contact affiliation	Spanish National Cancer Research Centre
contact email	jmunozpe@cnio.es
lab head
Ana Martinez
contact affiliation	Spanish National Cancer Research Centre
contact email	amdelval@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD003640

PRIDE project URI

• PRIDE
  1. PXD003640
     1. Label: PRIDE project
     2. Name: ON THE ACCURACY, PRECISION AND STATISTICAL SIGNIFICANCE OF COMPRESSED RATIOS IN ISOBARIC LABELLING