PXD003635

PXD003635 is an original dataset announced via ProteomeXchange.

Title	SCN circadian proteomes of miR-132/212 KO and WT mice by super-SILAC-based quantitative mass spectrometry
Description	To identify potential mechanisms underlying the phenotype of miR-132/212 KO mice under short photoperiods, we used quantitative mass spectrometry to analyze the SCN proteomes of miR-132/212 KO and WT mice under a 8:16 LD schedule at 4 time points, spaced 6h apart, across a 24h cycle (n=4 per time point per genotype). Quantification was achieved using a spike-in reference of three murine neural cell lines, including Neuro2A, and the adult mouse hypothalamic cell lines mHypoA-2/28 (CLU188) and mHypoA-SCN mix (CLU497) that had been labeled by SILAC (stable isotope labeling by amino acids in cell culture).
HostingRepository	PRIDE
AnnounceDate	2017-04-24
AnnouncementXML	Submission_2017-04-24_06:04:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kerwin chiang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-15 02:07:12	ID requested
⏵ 1	2017-04-24 06:04:52	announced

Mendoza-Viveros L, Chiang CK, Ong JLK, Hegazi S, Cheng AH, Bouchard-Cannon P, Fana M, Lowden C, Zhang P, Bothorel B, Michniewicz MG, Magill ST, Holmes MM, Goodman RH, Simonneaux V, Figeys D, Cheng HM, miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock. Cell Rep, 19(3):505-520(2017) [pubmed]

curator keyword: Biological

submitter keyword: mouse, SCN, LC-MS/MS

Daniel Figeys
contact affiliation	Ottawa Institute of Systems Biology and Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, ON, Canada
contact email	dfigeys@uottawa.ca
lab head
kerwin chiang
contact affiliation	university of ottawa
contact email	f94223019@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD003635

PRIDE project URI

• PRIDE
  1. PXD003635
     1. Label: PRIDE project
     2. Name: SCN circadian proteomes of miR-132/212 KO and WT mice by super-SILAC-based quantitative mass spectrometry