PXD003628

PXD003628 is an original dataset announced via ProteomeXchange.

Title	SNX27 interactome during immune synapse formation
Description	T lymphocyte recognition of antigenic peptides on the surface of antigen-presenting cells (APC) leads to immune synapse formation (IS). By analogy with the nervous synapse, this cell-cell communication model requires the formation of highly organized structures that delimit an active zone of membrane traffic. For T cells to connect antigen recognition with the biological response, receptors and signaling proteins must be recycled. Sorting nexin 27 (SNX27) is an endosomal protein best known for its role in recycling PDZ (PSD95, Dlg1, ZO-1)-interacting neuronal proteins via the retromer transport machinery. Alteration of this sorting pathway leads to neurodegeneration and is associated to Down syndrome, Alzheimer’s and Parkinson’s diseases. In T lymphocytes, SNX27 interacts with diacylglycerol kinase zeta (DGKz), which provides a mechanistic link between diacylglycerol metabolism and membrane trafficking. Following antigen recognition, SNX27-positive endosomes polarize to the IS and a PDZ-dependent SNX27 pool accumulates at the cell-cell contact area. To test for additional SNX27 functions, we carried out proteomic analysis of the SNX27 interactome purified from T cells in contact with APC, and identified SNX27 interaction with the retromer and with members of the WASH (Wiskott-Aldrich syndrome protein and SCAR homologue) complex. This finding indicates the conserved nature of the SNX27/WASH/retromer complex in hematopoietic cells and suggests similarity between neurological diseases and abnormal immune/inflammatory responses. We also found PDZ-dependent SNX27 cargoes, including modulators of cytoskeletal reorganization such as zona occludens-2 (ZO-2). ZO-2, a cytosolic component of epithelial cell-cell junctions, localized to the IS, and SNX27 silencing affected ZO-2 stability at the synapse. This study broadens our knowledge of SNX27 function in T lymphocytes, and suggests that pathways that delimit polarized structures in epithelial systems also participate in IS regulation.
HostingRepository	PRIDE
AnnounceDate	2017-05-11
AnnouncementXML	Submission_2017-05-11_04:15:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003628
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gonzalo Martínez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-15 00:45:24	ID requested
⏵ 1	2017-05-11 04:15:39	announced

Tello-Lafoz M, Mart, í, nez-Mart, í, nez G, Rodr, í, guez-Rodr, í, guez C, Albar JP, Huse M, Gharbi S, Merida I, Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse. Traffic, 18(8):491-504(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: SNX27, ZO-2, PDZ, immune synapse, T-lymphocyte

Isabel Mérida
contact affiliation	Dept of Immunology and Oncology, Centro Nacional de Biotecnología (CNB-CSIC), Darwin 3, Campus UAM Cantoblanco, 28079 Madrid, Spain
contact email	imerida@cnb.csic.es
lab head
Gonzalo Martínez
contact affiliation	Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Madrid, Spain
contact email	gmartinez@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003628
     1. Label: PRIDE project
     2. Name: SNX27 interactome during immune synapse formation