PXD003623

PXD003623 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nano-liquid chromatography-Orbitrap MS/MS
Description	A proteomics strategy based on nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The objectives of this study were (i) to detect changes in amplitude and kinetics of proteins altered by the treatment over the time; and (ii) to identify proteomic differences between the cytostatic and cytotoxic effect of the natural extract tested in order to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_01:11:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Valdés
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	4x(2)H labeled dimethylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-12 03:09:29	ID requested
1	2016-06-08 03:30:44	announced
2	2017-06-20 05:43:56	announced	Updated project metadata.
⏵ 3	2017-10-24 01:11:38	announced	Updated project metadata.

Vald, é, s A, Artemenko KA, Bergquist J, Garc, í, a-Ca, ñ, as V, Cifuentes A, Comprehensive Proteomic Study of the Antiproliferative Activity of a Polyphenol-Enriched Rosemary Extract on Colon Cancer Cells Using Nanoliquid Chromatography-Orbitrap MS/MS. J Proteome Res, 15(6):1971-85(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: antiproliferative activity, foodomics, colon cancer, dimethyl labeling, HT-29 cells, mass spectrometry, quantitative proteomics, rosemary extract

Alejandro Cifuentes
contact affiliation	Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Calle Nicolás Cabrera 9, 28049, Madrid, Spain.
contact email	a.cifuentes@csic.es
lab head
Alberto Valdés
contact affiliation	Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Calle Nicolás Cabrera 9, 28049, Madrid, Spain
contact email	a.valdes@csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003623

PRIDE project URI

• PRIDE
  1. PXD003623
     1. Label: PRIDE project
     2. Name: Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nano-liquid chromatography-Orbitrap MS/MS