PXD003614

PXD003614 is an original dataset announced via ProteomeXchange.

Title	Titration of Sall4 by the NuRD complex facilitates neural differentiation of ES cells
Description	Sall4 is a transcription factor essential for early mammalian development. Though it is reported to play an important role in embryonic stem (ES) cell self-renewal, whether it is an essential pluripotency factor has been disputed. Though Sall4 is known to associate with the Nucleosome Remodeling and Deacetylase (NuRD) complex, the nature of this interaction is unclear as NuRD and Sall4 serve opposing functions in ES cells. Here we use defined culture conditions and single-cell gene expression analyses to show that Sall4 prevents activation of the neural gene expression programme in ES cells but is dispensable for maintaining the pluripotency gene regulatory network. We further show using genome-wide analyses that while Sall4 interacts with NuRD, it neither recruits NuRD to chromatin nor influences transcription via NuRD. Rather we propose a model where, by titrating Sall4 protein, NuRD limits the differentiation-inhibiting activity of Sall4 in ES cells to enable lineage commitment.
HostingRepository	PRIDE
AnnounceDate	2016-08-02
AnnouncementXML	Submission_2016-08-02_07:37:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susan Kloet
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-11 02:18:13	ID requested
⏵ 1	2016-08-02 07:37:06	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: AP-MS of Flag-tagged Sall4 in mouse embryonic stem cells

Michiel Vermeulen
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, The Netherlands
contact email	m.vermeulen@ncmls.ru.nl
lab head
Susan Kloet
contact affiliation	Radboud University
contact email	s.kloet@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD003614

PRIDE project URI

• PRIDE
  1. PXD003614
     1. Label: PRIDE project
     2. Name: Titration of Sall4 by the NuRD complex facilitates neural differentiation of ES cells