21 proteins of three different size catgeories were purchased from a commercial supplier and used in different runs at different volumes. A yeast proteome background was present in every sample. This allows researchers to download the raw data, develop their own mathematical methods, and compare to others' methods when they upload their processed data to a Shiny application that calculates a range of performance measures. In the present study, different methods of summarising peptides to proteins and normalising between channels and between runs were compared.