PXD003604

PXD003604 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Efficient micro-scale basic reverse phase peptide fractionation for global and targeted proteomics
Description	This work describes a micro-scale basic reverse phase liquid chromatographic (bRPLC) fractionation method that offers high reproducibility and efficiency for peptide mixtures from small (5-20 g) samples. Shotgun and targeted proteomic analysis were performed to detect differentially expressed proteins from lung tumor cell lines that are sensitive (11-18) and resistant (11-18R) to the tyrosine kinase inhibitor erlotinib using micro-bRPLC.
HostingRepository	MassIVE
AnnounceDate	2019-10-03
AnnouncementXML	Submission_2019-10-03_08:34:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robbert Slebos
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-09 10:06:31	ID requested
⏵ 1	2019-10-03 08:34:15	announced

Publication List

Lee HJ, Kim HJ, Liebler DC, Efficient Microscale Basic Reverse Phase Peptide Fractionation for Global and Targeted Proteomics. J Proteome Res, 15(7):2346-54(2016) [pubmed]

Lee HJ, Kim HJ, Liebler DC, Efficient Microscale Basic Reverse Phase Peptide Fractionation for Global and Targeted Proteomics. J Proteome Res, 15(7):2346-54(2016) [pubmed]

Keyword List

submitter keyword: cell lines, colon tumor tissue

submitter keyword: cell lines, colon tumor tissue

Contact List

Daniel Liebler
lab head
Robbert Slebos
contact affiliation	Moffitt
contact email	robbert.slebos@moffitt.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079500/