PXD003599 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HVint validation by AP-MS analysis of HSV-1 VP26 |
Description | Human herpesviruses are widespread human pathogens with a remarkable impact on worldwide public health. Despite intense decades of research, the molecular details in many aspects of their function remain to be fully characterized. To unravel the details of how these viruses operate a thorough understanding of the relationships between the viral protein components is key. Here, we have created HVint, a novel protein-protein intra-viral interaction database for herpes simplex virus type 1 (HSV-1), which integrates data from five external sources. The coverage of the initial interactome was enlarged using evolutionary information, which integrates computational predictions for potential novel interactions in HSV-1. As an experimental validation for these predicted interactions, affinity purification mass spectrometry (AP-MS) was performed for the HSV-1 capsid protein, VP26. The VP26 interaction data provided experimental evidence supporting the interactions with pUL31 and pUL40, which were computationally predicted as direct partners. Other indirect interactions within the predicted network, such as pUL32 and pUL21, were also present in the AP-MS experiment. For access to the complete HVint network, we have developed a user-friendly and interactive web interface. Our approach demonstrates the power of computational predictions to assist in the design of targeted experiments for the discovery of novel protein-protein interactions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:27:45.085.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003599 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Todd Greco |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Ammonia-loss; Phospho; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-09 05:53:31 | ID requested | |
1 | 2016-07-15 00:04:43 | announced | |
2 | 2017-03-04 00:30:27 | announced | Updated project metadata. |
3 | 2017-10-24 04:07:03 | announced | Updated project metadata. |
⏵ 4 | 2024-10-22 04:27:46 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.M116.058552; |
Ashford P, Hernandez A, Greco TM, Buch A, Sodeik B, Cristea IM, Gr, ΓΌ, newald K, Shepherd A, Topf M, HVint: A Strategy for Identifying Novel Protein-Protein Interactions in Herpes Simplex Virus Type 1. Mol Cell Proteomics, 15(9):2939-53(2016) [pubmed] |
10.6019/PXD003599; |
Keyword List
submitter keyword: protein interactions, Orbitrap Velos, human, herpes simplex virus, virology, interactome |
Contact List
Ileana Cristea |
contact affiliation | Department of Molecular Biology, Princeton University |
contact email | icristea@princeton.edu |
lab head | |
Todd Greco |
contact affiliation | Molecular Biology, Princeton University |
contact email | tgreco@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD003599 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003599
- Label: PRIDE project
- Name: HVint validation by AP-MS analysis of HSV-1 VP26