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PXD003599

PXD003599 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHVint validation by AP-MS analysis of HSV-1 VP26
DescriptionHuman herpesviruses are widespread human pathogens with a remarkable impact on worldwide public health. Despite intense decades of research, the molecular details in many aspects of their function remain to be fully characterized. To unravel the details of how these viruses operate a thorough understanding of the relationships between the viral protein components is key. Here, we have created HVint, a novel protein-protein intra-viral interaction database for herpes simplex virus type 1 (HSV-1), which integrates data from five external sources. The coverage of the initial interactome was enlarged using evolutionary information, which integrates computational predictions for potential novel interactions in HSV-1. As an experimental validation for these predicted interactions, affinity purification mass spectrometry (AP-MS) was performed for the HSV-1 capsid protein, VP26. The VP26 interaction data provided experimental evidence supporting the interactions with pUL31 and pUL40, which were computationally predicted as direct partners. Other indirect interactions within the predicted network, such as pUL32 and pUL21, were also present in the AP-MS experiment. For access to the complete HVint network, we have developed a user-friendly and interactive web interface. Our approach demonstrates the power of computational predictions to assist in the design of targeted experiments for the discovery of novel protein-protein interactions.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:27:45.085.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003599
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTodd Greco
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListAmmonia-loss; Phospho; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-09 05:53:31ID requested
12016-07-15 00:04:43announced
22017-03-04 00:30:27announcedUpdated project metadata.
32017-10-24 04:07:03announcedUpdated project metadata.
42024-10-22 04:27:46announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.M116.058552;
Ashford P, Hernandez A, Greco TM, Buch A, Sodeik B, Cristea IM, Gr, ΓΌ, newald K, Shepherd A, Topf M, HVint: A Strategy for Identifying Novel Protein-Protein Interactions in Herpes Simplex Virus Type 1. Mol Cell Proteomics, 15(9):2939-53(2016) [pubmed]
10.6019/PXD003599;
Keyword List
submitter keyword: protein interactions, Orbitrap Velos, human, herpes simplex virus, virology, interactome
Contact List
Ileana Cristea
contact affiliationDepartment of Molecular Biology, Princeton University
contact emailicristea@princeton.edu
lab head
Todd Greco
contact affiliationMolecular Biology, Princeton University
contact emailtgreco@princeton.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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