PXD003598

PXD003598 is an original dataset announced via ProteomeXchange.

Title	Cyclin-dependant kinase (CDK) orders the cell cycle via substrate specific thresholds
Description	The appropriate ordering of the cell cycle events in eukaryoates is thought to be brought about by qualitative differences in the substrate specificity of multiple differentially expression Cyclin-CDK complexes. Our analysis of fission yeast supports an alternative quantitative model. Here we report a phosphoproteomics based global analysis of CDK substrate phosphorylation. We show that the phosphorylation of different CDK substrates is precisely ordered during the cell cycle by a single Cyclin-CDK complex. This is achieved by the differential sensitivity of substrates to a progressively rising CDK-to-phosphatase activity ratio, with early-phosphorylated substrates more sensitive to CDK activity than late substrates. This is combined with a rapid substrate phosphorylation turnover to generate clearly resolved substrate-specific thresholds, which in turn ensures the temporal ordering of downstream cell cycle events. These observations can also explain the major genetic redundancies between different cyclins and CDKs in higher eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2018-04-11
AnnouncementXML	Submission_2018-04-11_04:58:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Jones
SpeciesList	scientific name: Schizosaccharomyces pombe (Fission yeast); NCBI TaxID: 4896;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-09 05:19:21	ID requested
⏵ 1	2018-04-11 04:58:52	announced

Swaffer MP, Jones AW, Flynn HR, Snijders AP, Nurse P, CDK Substrate Phosphorylation and Ordering the Cell Cycle. Cell, 167(7):1750-1761.e16(2016) [pubmed]

submitter keyword: SILAC, mass spectrometry, cell cycle, S. pombe, CDK

Ambrosius P. Snijders
contact affiliation	The Francis Crick Institute
contact email	Bram.Snijders@crick.ac.uk
lab head
Andrew Jones
contact affiliation	The Francis Crick Institute
contact email	Andrew.Jones@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD003598

PRIDE project URI

• PRIDE
  1. PXD003598
     1. Label: PRIDE project
     2. Name: Cyclin-dependant kinase (CDK) orders the cell cycle via substrate specific thresholds