PXD003596

PXD003596 is an original dataset announced via ProteomeXchange.

Title	Inhibitory effect of miR-183 on MCM family members
Description	MYCN and HDAC2 jointly repress the transcription of tumor suppressive micro RNA miR-183 in neuroblastoma. Enforced miR-183 expression induces neuroblastoma cell death and inhibits anchorage-independent colony formation and subcutaneous xenograft growth in mice. We here aimed to unravel the miR-183 signaling network and elucidated the role of MYCN mediated transcriptional activation of members of the minichromosome maintenance (MCM) family protein family involving miR-183 . The hexamer protein complex formed by MCM proteins is involved in the initiation and elongation of eukaryotic genome replication, thereby contributing to genomic integrity. Analysis of miR-183 versus negative control transfected neuroblastoma cells identified 85 differentially expressed proteins in a label-free mass spectrometric approach. Six members of the MCM family were found to be lower expressed upon enforced miR-183 expression, and subsequent annotation category enrichment analysis revealed a 14-fold enrichment in the protein module category “MCM”. Down-regulation was confirmed by western blot analysis. MicroRNA target prediction software studies revealed that miR-183 was predicted to directly target several MCMs.
HostingRepository	PRIDE
AnnounceDate	2016-06-01
AnnouncementXML	Submission_2016-06-01_01:19:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-09 03:21:21	ID requested
⏵ 1	2016-06-01 01:19:11	announced

Lodrini M, Poschmann G, Schmidt V, W, ü, nschel J, Dreidax D, Witt O, H, ö, fer T, Meyer HE, St, ü, hler K, Eggert A, Deubzer HE, Minichromosome Maintenance Complex Is a Critical Node in the miR-183 Signaling Network of MYCN-Amplified Neuroblastoma Cells. J Proteome Res, 15(7):2178-86(2016) [pubmed]

curator keyword: Biological

submitter keyword: cell cycle progression, eukaryotic genome replication, genomic integrity, label-free proteomics, oncogene

Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory (MPL) Biologisch-Medizinisches Forschungszentrum (BMFZ) Heinrich-Heine-Universität Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003596
     1. Label: PRIDE project
     2. Name: Inhibitory effect of miR-183 on MCM family members