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PXD003594

PXD003594 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn increase in oxidative stress downstream of mTORC1 but not AKT causes a proliferative defect in cancer cells with acquired resistance to PI3K/mTOR inhibitors
DescriptionProteomics and phosphoproteomics analysis of MCF7 cells sensitive and resistant to the PI3K inhibitor GDC-0941. The analysis was done for parental and 3 resistant cell lines maintained in the presence or the absence of the drug. One of the resistant cell lines (G2) was also treated with vehicle or the kinase inhibitors GDC0941, MK-2206 and Ku-0063794 for 2h. The proteomics analysis of sensitive and resistant MCF7 cells in basal conditions and phosphoproteomics analysis of the G2 cells in the presence of inhibitors of the PI3K pathway were run in a nano flow ultrahigh pressure liquid chromatography (UPLC, nano Acquity, Waters) coupled to an LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific). The phosphoproteomics study of sensitive and resistant MCF7 cells in basal conditions was run in a Dionex UltiMate 3000 RSLCnano coupled to an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific).
HostingRepositoryPRIDE
AnnounceDate2018-04-09
AnnouncementXMLSubmission_2018-04-09_02:24:11.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003594
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPedro Casado-Izquierdo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
InstrumentQ Exactive; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-09 02:02:51ID requested
12018-04-09 02:24:12announced
Publication List
Dermit M, Casado P, Rajeeve V, Wilkes EH, Foxler DE, Campbell H, Critchlow S, Sharp TV, Gribben JG, Unwin R, Cutillas PR, Oxidative stress downstream of mTORC1 but not AKT causes a proliferative defect in cancer cells resistant to PI3K inhibition. Oncogene, 36(19):2762-2774(2017) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, PI3K resistance, Metabolism, LTQ-Orbitrap, Q-Exactive Plus, Phosphoproteomics, TiO2
Contact List
Pedro R. Cutillas
contact affiliationBarts Cancer Institute
contact emailp.cutillas@qmul.ac.uk
lab head
Pedro Casado-Izquierdo
contact affiliationCell Signalling
contact emailp.m.casado-izquierdo@qmul.ac.uk
dataset submitter
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Dataset FTP location
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