PXD003587

PXD003587 is an original dataset announced via ProteomeXchange.

Title	Acetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids.
Description	In this project we have studied human hepatoma cell line grown in 3D spheroids subjected to APAP (acetaminophen) treatment. Global protein expression analysis (label free) and analysis of cysteine oxidations (SNO/SOH TMT proteomics) have been combined to get insights in to the molecular response triggered.
HostingRepository	PRIDE
AnnounceDate	2018-10-18
AnnouncementXML	Submission_2018-10-26_00:39:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adelina Rogowska-Wrzesinska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	cysTMT6plex reporter+balance reagent cysteine disulfide; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-08 03:37:59	ID requested
1	2018-10-18 11:36:45	announced
⏵ 2	2018-10-26 00:40:00	announced	Updated publication reference for PubMed record(s): 30090399.

Wojdyla K, Wrzesinski K, Williamson J, Fey SJ, Rogowska-Wrzesinska A, -sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids. Toxicol Res (Camb), 5(3):905-920(2016) [pubmed]

submitter keyword: APAP

3D cell culture

S-nitrosylation

S-sulfenylation

quantitative redox proteomics

toxicology

HepG2/C3A

Adelina Rogowska-Wrzesinska
contact affiliation	Protein Research Group Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
contact email	adelinar@bmb.sdu.dk
lab head
Adelina Rogowska-Wrzesinska
contact affiliation	University of Southern Denmark
contact email	adelinar@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003587

PRIDE project URI

• PRIDE
  1. PXD003587
     1. Label: PRIDE project
     2. Name: Acetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids.