PXD003577 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Open-Gate Mutants of the Mammalian Proteasome Show Enhanced Ubiquitin-Conjugate Degradation |
Description | When in the closed form, the axial substrate translocation channel of the proteasome core particle (CP) is topologically blocked by the convergent N-termini of subunits. To probe the role of channel gating in mammalian proteasomes, we have deleted the N-terminal tail of 3. The resulting 3N proteasomes are intact but hyperactive in the hydrolysis of fluorogenic peptide substrates and degradation of polyubiquitinated Sic1. Cells expressing the hyperactive proteasomes show markedly elevated degradation of established proteasome substrates and resistance to oxidative stress, and multiplexed quantitative proteomics reveal ~ 200 proteins with reduced levels in the mutant cells. Potentially toxic proteins such as tau exhibit reduced accumulation and aggregate formation. These data demonstrate that the CP gate is a key negative regulator of proteasome function in mammals, and that opening the CP gate may be an effective strategy to increase proteasome activity and reduce levels of toxic proteins in cells. |
HostingRepository | PRIDE |
AnnounceDate | 2016-03-01 |
AnnouncementXML | Submission_2016-03-11_00:04:12.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Min Jae Lee |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-04 01:58:54 | ID requested | |
1 | 2016-03-01 01:29:36 | announced | |
⏵ 2 | 2016-03-11 00:04:13 | announced | Updated publication reference for PubMed record(s): 26957043. |
Publication List
Choi WH, de Poot SA, Lee JH, Kim JH, Han DH, Kim YK, Finley D, Lee MJ, Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation. Nat Commun, 7():10963(2016) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: proteasome, gate, tau, aggregation, oxidative stress, proteomics |
Contact List
Min Jae Lee |
contact affiliation | Department of Biomedical Sciences, Seoul National University Graduate School, Seoul 03080, Korea. |
contact email | minjlee@snu.ac.kr |
lab head | |
Min Jae Lee |
contact affiliation | Seoul National University College of Medicine |
contact email | minjlee@snu.ac.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003577
- Label: PRIDE project
- Name: Open-Gate Mutants of the Mammalian Proteasome Show Enhanced Ubiquitin-Conjugate Degradation