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PXD003577

PXD003577 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOpen-Gate Mutants of the Mammalian Proteasome Show Enhanced Ubiquitin-Conjugate Degradation
DescriptionWhen in the closed form, the axial substrate translocation channel of the proteasome core particle (CP) is topologically blocked by the convergent N-termini of  subunits. To probe the role of channel gating in mammalian proteasomes, we have deleted the N-terminal tail of 3. The resulting 3N proteasomes are intact but hyperactive in the hydrolysis of fluorogenic peptide substrates and degradation of polyubiquitinated Sic1. Cells expressing the hyperactive proteasomes show markedly elevated degradation of established proteasome substrates and resistance to oxidative stress, and multiplexed quantitative proteomics reveal ~ 200 proteins with reduced levels in the mutant cells. Potentially toxic proteins such as tau exhibit reduced accumulation and aggregate formation. These data demonstrate that the CP gate is a key negative regulator of proteasome function in mammals, and that opening the CP gate may be an effective strategy to increase proteasome activity and reduce levels of toxic proteins in cells.
HostingRepositoryPRIDE
AnnounceDate2016-03-01
AnnouncementXMLSubmission_2016-03-11_00:04:12.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMin Jae Lee
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcarbamoylated residue; TMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-04 01:58:54ID requested
12016-03-01 01:29:36announced
22016-03-11 00:04:13announcedUpdated publication reference for PubMed record(s): 26957043.
Publication List
Choi WH, de Poot SA, Lee JH, Kim JH, Han DH, Kim YK, Finley D, Lee MJ, Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation. Nat Commun, 7():10963(2016) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: proteasome, gate, tau, aggregation, oxidative stress, proteomics
Contact List
Min Jae Lee
contact affiliationDepartment of Biomedical Sciences, Seoul National University Graduate School, Seoul 03080, Korea.
contact emailminjlee@snu.ac.kr
lab head
Min Jae Lee
contact affiliationSeoul National University College of Medicine
contact emailminjlee@snu.ac.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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