PXD003573

PXD003573 is an original dataset announced via ProteomeXchange.

Title	The Rab-binding profiles of Legionella effectors
Description	Legionella pneumophila, the causative agent of Legionnaire’s disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the roles of most of the effectors are yet to be characterized. Key to understanding the function of effectors is the identification of host proteins they bind during infection. We previously developed a novel tandem-affinity purification (TAP) approach using hexahistidine and BirA-specific biotinylation tags for isolating translocated effector complexes from infected cells whose composition were subsequently deciphered by mass spectrometry. Here we further advanced the workflow for the TAP approach and determined the infection-dependent interactomes of the effectors SidM and LidA, which were previously reported to promiscuously bind multiple Rab GTPases in vitro. In this study we defined a stringent subset of Rab GTPases targeted by SidM and LidA during infection, comprising of Rab1A, 1B, 6 and 10; in addition, LidA targets Rab14 and 18. Taken together, this study illustrates the power of this approach to profile the intracellular interactomes of bacterial effectors during infection.
HostingRepository	PRIDE
AnnounceDate	2016-02-11
AnnouncementXML	Submission_2016-02-11_03:15:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ernest So
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-04 01:15:40	ID requested
⏵ 1	2016-02-11 03:15:52	announced

So EC, Schroeder GN, Carson D, Mattheis C, Mousnier A, Broncel M, Tate EW, Frankel G, The Rab-binding Profiles of Bacterial Virulence Factors during Infection. J Biol Chem, 291(11):5832-5843(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Rab GTPase, Legionella, effector

Gad Frankel
contact affiliation	MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, UK
contact email	g.frankel@imperial.ac.uk
lab head
Ernest So
contact affiliation	Imperial College London
contact email	ecs11@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD003573

PRIDE project URI

• PRIDE
  1. PXD003573
     1. Label: PRIDE project
     2. Name: The Rab-binding profiles of Legionella effectors