PXD003570

PXD003570 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based proteomic analysis reveals genome-wide protein expression patterns in allohexaploid bread wheat in adaptation to potassium deficiency and an important role of jasmonic acid
Description	Two-week old bread wheat seedlings hydroponically grown Hoagland solution were transferred to potassium (K+)-free conditions for 8 d, their root and leaf proteome profiles were assessed using iTRAQ proteome method, and NCBInr database combined with the recently published bread wheat genome information were used to analyze the identified protein species. Over 4,000 unique proteins were identified, 818 K+-responsive protein species showed significant abundance regulation. The most majority of the identified K+-responsive protein species had exact gene loci, and showed no global but tissue- and chromosome- dependent genome distributions. The identified protein species were associated with diverse functions and exhibited organ-specific differences. Most of identified protein species associated with hormone synthesis were the enzymes involved in the synthesis of jasmonic acid (JA). Allene oxide synthase (AOS), a key JA synthesis-related enzyme, was significantly induced in both root and leaf organs of K+-deficient wheat seedlings, and its overexpression in rice enhanced the tolerance to low K+ or K+ deficiency, increased contents of K+ and JA and transcription levels of some K+-responsive genes. However, rice AOS T-DNA inserted mutant (osaos) exhibited more sensitivity to K+ deficiency. K+ deficiency significantly increased abundance of a high affinity K+ transporter (TaAHAK1), TaAHAK1 transgenic rice seedlings markedly alleviated sensitivity to K+ deficiency, and K+ deficiency also upregulated expression of homologous OsHAK1 gene in TaAOS transgenic rice plants. These results suggested an essential role of JA in K+ deficiency and gave molecular insight into the responses of plant to K+ deficiency.
HostingRepository	PRIDE
AnnounceDate	2017-10-09
AnnouncementXML	Submission_2017-10-09_02:25:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gezi Li
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-03 07:43:34	ID requested
⏵ 1	2017-10-09 02:25:21	announced

Li G, Wu Y, Liu G, Xiao X, Wang P, Gao T, Xu M, Han Q, Wang Y, Guo T, Kang G, Large-scale Proteomics Combined with Transgenic Experiments Demonstrates An Important Role of Jasmonic Acid in Potassium Deficiency Response in Wheat and Rice. Mol Cell Proteomics, 16(11):1889-1905(2017) [pubmed]

curator keyword: Biological

submitter keyword: iTRAQ, jasmonic acid, potassium deficiency, Triticum aestivum L.

Guozhang Kang
contact affiliation	Henan Agricultural University
contact email	zkangg@163.com
lab head
Gezi Li
contact affiliation	Henan Agricultural University
contact email	gezi888@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003570
     1. Label: PRIDE project
     2. Name: iTRAQ-based proteomic analysis reveals genome-wide protein expression patterns in allohexaploid bread wheat in adaptation to potassium deficiency and an important role of jasmonic acid