PXD003553

PXD003553 is an original dataset announced via ProteomeXchange.

Title	Distinct Combinatorial Cascade of Events Generated by ECM Proteolysis Dictates Cell Behavior
Description	It is well established that the expression profiles of multiple and possibly redundant matrix remodeling proteases (e.g. collagenases) strongly differentiates in disease and development. Although enzymatic redundancy might be inferred from their close similarity in structure, their in-vivo activity can lead to extremely diverse tissue-remodeling outcomes. We observed that proteolysis of collagen, generated uniquely by individual homologous proteases, leads to a specific cascade of combinatorial events, which eventually affects overall extracellular matrix (ECM) topography, visco-elastic properties and composition. We reveal striking differences in the migratory and signaling patterns, morphology, and gene expression profiles of cells interacting with native collagen-rich ECM matrix degraded by different collagenases. Thus unlike envisioned before degradative matrix remodeling systems are not redundant and give rise to precise ECM-cell crosstalk. As ECM proteolysis is an abundant biochemical process critical to tissue homeostasis these results improve our fundamental understanding of systemic factors dictating cell behavior.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_11:59:21.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yishai Levin
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-01 05:36:50	ID requested
⏵ 1	2021-03-04 11:59:22	announced

Solomonov I, Zehorai E, Talmi-Frank D, Wolf SG, Shainskaya A, Zhuravlev A, Kartvelishvily E, Visse R, Levin Y, Kampf N, Jaitin DA, David E, Amit I, Nagase H, Sagi I, Distinct biological events generated by ECM proteolysis by two homologous collagenases. Proc Natl Acad Sci U S A, 113(39):10884-9(2016) [pubmed]

curator keyword: Biological

submitter keyword: ECM, proteomics, metalloproteinase

Irit Sagi
contact affiliation	Weizmann Institute of Science
contact email	irit.sagi@weizmann.ac.il
lab head
Yishai Levin
contact affiliation	Weizmann Institute of Science, Israel
contact email	yishai.levin@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD003553

PRIDE project URI

• PRIDE
  1. PXD003553
     1. Label: PRIDE project
     2. Name: Distinct Combinatorial Cascade of Events Generated by ECM Proteolysis Dictates Cell Behavior