PXD003551

PXD003551 is an original dataset announced via ProteomeXchange.

Title	Ki-67 organises heterochromatin during cell proliferation
Description	Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in differentiated tissues did not inhibit cell cycle arrest. Ki-67 interactors included proteins involved in nucleolar processes and chromatin regulators. Ki-67 depletion disrupted nucleologenesis but did not inhibit pre-rRNA processing. In contrast, it altered gene expression. Ki-67 silencing also had wide-ranging effects on chromatin organisation, disrupting heterochromatin compaction and long-range genomic interactions. Trimethylation of histone H3K9 and H4K20 at heterochromatin was strongly reduced. Overexpression of human or Xenopus Ki-67 induced ectopic heterochromatin formation. Altogether, our results suggest that Ki-67 expression in proliferating cells spatially organises heterochromatin, thereby controlling gene expression.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_00:56:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003551
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	sonia hem
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-01 02:35:22	ID requested
⏵ 1	2018-10-26 00:56:18	announced

Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Ll, è, res D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, D, é, jardin J, Malumbres M, Jay P, Dulic V, Lafontaine DLj, Feil R, Fisher D, The cell proliferation antigen Ki-67 organises heterochromatin. Elife, 5():e13722(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: MKI67, antigen ki-67, ki-67 interactome, cell proliferation, heterochromatin

Daniel Fisher
contact affiliation	Montpellier Institute of Molecular Genetics, IGMM, CNRS UMR 5535, 34293 Montpellier, France University of Montpellier, Faculty of Sciences, 34095 Montpellier, France
contact email	daniel.fisher@igmm.cnrs.fr
lab head
sonia hem
contact affiliation	INRA
contact email	sonia.hem@supagro.inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003551

PRIDE project URI

• PRIDE
  1. PXD003551
     1. Label: PRIDE project
     2. Name: Ki-67 organises heterochromatin during cell proliferation