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PXD003550

PXD003550 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome analysis of adipose-derived stem cells cultured under clinically relevant conditions in a wound healing perspective
DescriptionThere is a large body of evidence supporting the beneficial role of adipose-derived stem cells (ASCs) in tissue repair and regeneration. These effects appear to be mainly mediated by paracrine signaling pathways and enhanced during hypoxia. Mass spectrometry (MS) represents a valuable tool for proteomic profiling of cultured ASCs, which may help revealing the identity of the factors secreted by the cells under different conditions. However, serum starvation essentially required to obtain samples compatible with secretome analysis by MS can have significant influence on ASCs. Here, we present a novel and optimized culturing approach based on the use of a clinically relevant serum-free formulation, which was used to assess the effect of hypoxia on the ASC proteomic profile.
HostingRepositoryPRIDE
AnnounceDate2016-04-18
AnnouncementXMLSubmission_2016-04-18_08:15:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAllan Stensballe
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-01 02:17:42ID requested
12016-04-18 08:15:33announced
Publication List
Riis S, Stensballe A, Emmersen J, Pennisi CP, Birkelund S, Zachar V, Fink T, Mass spectrometry analysis of adipose-derived stem cells reveals a significant effect of hypoxia on pathways regulating extracellular matrix. Stem Cell Res Ther, 7(1):52(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Adipose-derived stem cells, proteomics, hypoxia, serum-free culture, secretome
Contact List
Allan Stensballe
contact affiliationLaboratory for Medical Mass spectrometry Aalborg University Fredrik Bajersvej 3B 9220 Aalborg
contact emailas@hst.aau.dk
lab head
Allan Stensballe
contact affiliationAalborg University
contact emailas@hst.aau.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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