PXD003550 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome analysis of adipose-derived stem cells cultured under clinically relevant conditions in a wound healing perspective |
Description | There is a large body of evidence supporting the beneficial role of adipose-derived stem cells (ASCs) in tissue repair and regeneration. These effects appear to be mainly mediated by paracrine signaling pathways and enhanced during hypoxia. Mass spectrometry (MS) represents a valuable tool for proteomic profiling of cultured ASCs, which may help revealing the identity of the factors secreted by the cells under different conditions. However, serum starvation essentially required to obtain samples compatible with secretome analysis by MS can have significant influence on ASCs. Here, we present a novel and optimized culturing approach based on the use of a clinically relevant serum-free formulation, which was used to assess the effect of hypoxia on the ASC proteomic profile. |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-18 |
AnnouncementXML | Submission_2016-04-18_08:15:32.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Allan Stensballe |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-01 02:17:42 | ID requested | |
⏵ 1 | 2016-04-18 08:15:33 | announced | |
Publication List
Riis S, Stensballe A, Emmersen J, Pennisi CP, Birkelund S, Zachar V, Fink T, Mass spectrometry analysis of adipose-derived stem cells reveals a significant effect of hypoxia on pathways regulating extracellular matrix. Stem Cell Res Ther, 7(1):52(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Adipose-derived stem cells, proteomics, hypoxia, serum-free culture, secretome |
Contact List
Allan Stensballe |
contact affiliation | Laboratory for Medical Mass spectrometry Aalborg University Fredrik Bajersvej 3B 9220 Aalborg |
contact email | as@hst.aau.dk |
lab head | |
Allan Stensballe |
contact affiliation | Aalborg University |
contact email | as@hst.aau.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003550 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003550
- Label: PRIDE project
- Name: Proteome analysis of adipose-derived stem cells cultured under clinically relevant conditions in a wound healing perspective