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PXD003545

PXD003545 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFatty-acylation in RAW264.7 - Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins
DescriptionDietary unsaturated fatty acids beneficially affect human health, in part by modulating the immune system, but the mechanism is not completely understood. Given that unsaturated fatty acids have been shown to be covalently incorporated into a small subset of proteins, we designed three alkyne-tagged chemical reporters of unsaturated fatty acids, alk-16:1, alk-17:1 and alk-18:1, to explore the generality and diversity of this protein modification. Following cell lysis, proteins labelled with the reporters could be captured by azido-functionalized reagents via CuAAC for fluorescence detection or enrichment for proteomics analysis. These reporters label many proteins in mammalian cells and can be incorporated site-specifically, notably on Cys residues. Quantitative proteomics analysis (n= 4 biological replicates) of LPS/IFN-gamma stimulated RAW264.7 labelled with oleic acid (control), alk-16 (palmitic acid chemical reporter), alk-16:1, alk-17:1 and alk-18:1, revealed that unsaturated fatty acids modify similar protein targets to saturated fatty acids, including several immune proteins. Interestingly, some proteins can be differentially labeled with unsaturated and saturated fatty acid.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:28:16.890.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEmmanuelle Thinon
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-29 01:58:08ID requested
12018-10-26 05:22:40announced
22024-10-22 04:28:24announced2024-10-22: Updated project metadata.
Publication List
10.1002/cbic.201600213;
Thinon E, Percher A, Hang HC, Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins. Chembiochem, 17(19):1800-1803(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: LC-MSMS,RAW264.7, unsaturated fatty acid, fatty-acylation
Contact List
Howard C. Hang
contact affiliationLaboratory of Chemical Biology and Microbial Pathogenesis The Rockefeller University USA
contact emailhhang@rockefeller.edu
lab head
Emmanuelle Thinon
contact affiliationThe Francis Crick Institute
contact emailemmanuellethinon@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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