PXD003545

PXD003545 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Fatty-acylation in RAW264.7 - Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins
Description	Dietary unsaturated fatty acids beneficially affect human health, in part by modulating the immune system, but the mechanism is not completely understood. Given that unsaturated fatty acids have been shown to be covalently incorporated into a small subset of proteins, we designed three alkyne-tagged chemical reporters of unsaturated fatty acids, alk-16:1, alk-17:1 and alk-18:1, to explore the generality and diversity of this protein modification. Following cell lysis, proteins labelled with the reporters could be captured by azido-functionalized reagents via CuAAC for fluorescence detection or enrichment for proteomics analysis. These reporters label many proteins in mammalian cells and can be incorporated site-specifically, notably on Cys residues. Quantitative proteomics analysis (n= 4 biological replicates) of LPS/IFN-gamma stimulated RAW264.7 labelled with oleic acid (control), alk-16 (palmitic acid chemical reporter), alk-16:1, alk-17:1 and alk-18:1, revealed that unsaturated fatty acids modify similar protein targets to saturated fatty acids, including several immune proteins. Interestingly, some proteins can be differentially labeled with unsaturated and saturated fatty acid.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:16.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuelle Thinon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-29 01:58:08	ID requested
1	2018-10-26 05:22:40	announced
⏵ 2	2024-10-22 04:28:24	announced	2024-10-22: Updated project metadata.

Publication List

10.1002/cbic.201600213;
Thinon E, Percher A, Hang HC, Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins. Chembiochem, 17(19):1800-1803(2016) [pubmed]

10.1002/cbic.201600213;

Thinon E, Percher A, Hang HC, Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins. Chembiochem, 17(19):1800-1803(2016) [pubmed]

Keyword List

curator keyword: Biomedical
submitter keyword: LC-MSMS,RAW264.7, unsaturated fatty acid, fatty-acylation

curator keyword: Biomedical

submitter keyword: LC-MSMS,RAW264.7, unsaturated fatty acid, fatty-acylation

Contact List

Howard C. Hang
contact affiliation	Laboratory of Chemical Biology and Microbial Pathogenesis The Rockefeller University USA
contact email	hhang@rockefeller.edu
lab head
Emmanuelle Thinon
contact affiliation	The Francis Crick Institute
contact email	emmanuellethinon@gmail.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003545

PRIDE project URI

Repository Record List

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