PXD003521 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Cypermethrin Induced Proteins Changes in Green Algae by iTRAQ Quantitative Proteomics |
Description | Cypermethrin (CYP) is one of the most widely used pesticides in large scale for agricultural and domestic purpose and the residue often seriously affects aquatic system. Environmental pollutants induced protein changes in organisms could be detected by proteomics, leading to discovery of potential biomarkers and understanding of mode of action. While proteomics investigations of CYP stress in some animal models have been well studied, few reports about the effects of exposure to CYP on algae proteome were published. To determine CYP effect in algae, the impact of various dosages (0.001 µg/L, 0.01 µg/L and 1 µg/L) of CYP on green algae Chlorella Vulgaris for 24h and 96h were investigated by using iTRAQ quantitative proteomics technique. A total of 162 and 198 proteins were significantly altered after CYP exposure for 24h and 96h, respectively. Overview of iTRAQ results indicated that the influence of CYP on algae protein might be dosage-dependent. Functional analysis of differentially expressed proteins showed that CYP could induce protein alterations related to photosynthesis, stress responses and carbohydrates metabolism. This study provides a comprehensive view of complex mode of action of algae under CYP stress and highlights several potential biomarkers for further investigation of pesticides exposed plant and algae. |
HostingRepository | PRIDE |
AnnounceDate | 2016-03-14 |
AnnouncementXML | Submission_2016-03-14_05:45:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yan Gao |
SpeciesList | scientific name: Chlorella vulgaris (Green alga); NCBI TaxID: 3077; |
ModificationList | deamidated residue; L-cysteine residue; monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-01-25 06:46:33 | ID requested | |
⏵ 1 | 2016-03-14 05:45:57 | announced | |
Publication List
Gao Y, Lim TK, Lin Q, Li SF, Identification of cypermethrin induced protein changes in green algae by iTRAQ quantitative proteomics. J Proteomics, 139():67-76(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Algae, iTRAQ, cypermethrin, photosynthesis, carbohydrate metabolism, stress responsive |
Contact List
LI Fong Yau Sam |
contact affiliation | Department of Chemistry, National University of Singapore, Singpaore |
contact email | chmlifys@nus.edu.sg |
lab head | |
Yan Gao |
contact affiliation | National University of Singapore |
contact email | a0068387@u.nus.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003521
- Label: PRIDE project
- Name: Identification of Cypermethrin Induced Proteins Changes in Green Algae by iTRAQ Quantitative Proteomics