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PXD003521

PXD003521 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Cypermethrin Induced Proteins Changes in Green Algae by iTRAQ Quantitative Proteomics
DescriptionCypermethrin (CYP) is one of the most widely used pesticides in large scale for agricultural and domestic purpose and the residue often seriously affects aquatic system. Environmental pollutants induced protein changes in organisms could be detected by proteomics, leading to discovery of potential biomarkers and understanding of mode of action. While proteomics investigations of CYP stress in some animal models have been well studied, few reports about the effects of exposure to CYP on algae proteome were published. To determine CYP effect in algae, the impact of various dosages (0.001 µg/L, 0.01 µg/L and 1 µg/L) of CYP on green algae Chlorella Vulgaris for 24h and 96h were investigated by using iTRAQ quantitative proteomics technique. A total of 162 and 198 proteins were significantly altered after CYP exposure for 24h and 96h, respectively. Overview of iTRAQ results indicated that the influence of CYP on algae protein might be dosage-dependent. Functional analysis of differentially expressed proteins showed that CYP could induce protein alterations related to photosynthesis, stress responses and carbohydrates metabolism. This study provides a comprehensive view of complex mode of action of algae under CYP stress and highlights several potential biomarkers for further investigation of pesticides exposed plant and algae.
HostingRepositoryPRIDE
AnnounceDate2016-03-14
AnnouncementXMLSubmission_2016-03-14_05:45:55.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYan Gao
SpeciesList scientific name: Chlorella vulgaris (Green alga); NCBI TaxID: 3077;
ModificationListdeamidated residue; L-cysteine residue; monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-25 06:46:33ID requested
12016-03-14 05:45:57announced
Publication List
Gao Y, Lim TK, Lin Q, Li SF, Identification of cypermethrin induced protein changes in green algae by iTRAQ quantitative proteomics. J Proteomics, 139():67-76(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Algae, iTRAQ, cypermethrin, photosynthesis, carbohydrate metabolism, stress responsive
Contact List
LI Fong Yau Sam
contact affiliationDepartment of Chemistry, National University of Singapore, Singpaore
contact emailchmlifys@nus.edu.sg
lab head
Yan Gao
contact affiliationNational University of Singapore
contact emaila0068387@u.nus.edu
dataset submitter
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Dataset FTP location
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