PXD003516

PXD003516 is an original dataset announced via ProteomeXchange.

Title	Proteomic insight into the response of Arabidopsis chloroplasts to darkness
Description	Chloroplast function in photosynthesis is essential for plant growth and development. It is well-known that chloroplasts respond to various light conditions, however, it remains poorly understood about how chloroplasts respond to darkness. In this study, we found 90 darkness-responsive proteins in Arabidopsis chloroplasts under 8 h darkness treatment. Most of the proteins are nucleus-encoded, indicating that chloroplast darkness response is closely regulated by the nucleus. Among them, 17 ribosome proteins were obviously reduced after darkness treatment. The protein expressional patterns and physiological characters revealed the mechanisms in chloroplasts in response to darkness, e.g., (1) inhibition of photosystem II resulted in preferential cyclic electron flow around PSI; (2) promotion of starch degradation; (3) inhibition of chloroplastic translation; and (4) regulation by redox and jasmonate signaling. The results have improved our understanding of molecular regulatory mechanisms in chloroplasts under darkness.
HostingRepository	PRIDE
AnnounceDate	2016-04-20
AnnouncementXML	Submission_2016-05-06_06:51:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaojun Dai
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-25 01:52:37	ID requested
1	2016-04-20 03:51:24	announced
⏵ 2	2016-05-06 06:51:18	announced	Updated publication reference for PubMed record(s): 27137770.

Wang J, Yu Q, Xiong H, Wang J, Chen S, Yang Z, Dai S, Proteomic Insight into the Response of Arabidopsis Chloroplasts to Darkness. PLoS One, 11(5):e0154235(2016) [pubmed]

curator keyword: Biological

submitter keyword: Chloroplast

Darkness

Proteomics

Arabidopsis

Molecular mechanisms

Shaojun Dai
contact affiliation	Shanghai Normal University
contact email	daishaojun@hotmail.com
lab head
Shaojun Dai
contact affiliation	NEFU
contact email	735425617@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003516

PRIDE project URI

• PRIDE
  1. PXD003516
     1. Label: PRIDE project
     2. Name: Proteomic insight into the response of Arabidopsis chloroplasts to darkness