PXD003514

PXD003514 is an original dataset announced via ProteomeXchange.

Title	SILAC-based quantitative Nox4-Co-immunoprecipitation
Description	Within the family of NADPH oxidases, Nox4 is unique as it is predominantly localized in the endoplasmic reticulum, has constitutive activity and generates H2O2. We hypothesize that these features are consequences of a so far unidentified Nox4-interacting protein. Interacting proteins were screened by quantitative SILAC-Co-immunoprecipitation in HEK293 cells stably overexpressing Nox4. By this technique, several interacting proteins were identified with calnexin showing the most robust interaction.
HostingRepository	PRIDE
AnnounceDate	2016-02-17
AnnouncementXML	Submission_2016-02-16_23:59:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-25 00:20:32	ID requested
⏵ 1	2016-02-17 00:00:00	announced

Prior KK, Wittig I, Leisegang MS, Groenendyk J, Weissmann N, Michalak M, Jansen-D, ü, rr P, Shah AM, Brandes RP, The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein. J Biol Chem, 291(13):7045-59(2016) [pubmed]

curator keyword: Biological

submitter keyword: SILAC, LC-MSMS, macromolecular complexes, Co-immunoprecipitation

Ralf Brandes
contact affiliation	Institut für Kardiovaskuläre Physiologie, Goethe-Universität, Frankfurt am Main, Germany
contact email	brandes@vrc.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD003514

PRIDE project URI

• PRIDE
  1. PXD003514
     1. Label: PRIDE project
     2. Name: SILAC-based quantitative Nox4-Co-immunoprecipitation