PXD003485

PXD003485 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Detergents assisted glycoprotein capture
Description	A detergents assisted glycoprotein capture method was developed to increase the coverage of N-glycoproteome of various samples.Application of this approach in the larger scale N-glycoproteomics analysis of the HEK 293 cell membrane led to the identification of 2253 unique N-glycosites from 953 proteins. Application of this approach to human serum resulted in the identification of 850 N-glycosylation sites without any immuno-depletion and fractionation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:27:42.657.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-19 01:47:02	ID requested
1	2016-12-23 07:33:18	announced
2	2017-10-24 03:41:10	announced	Updated project metadata.
⏵ 3	2024-10-22 04:27:47	announced	2024-10-22: Updated project metadata.

Publication List

10.1021/acs.jproteome.6b00056;
Chen R, Zou H, Figeys D, Detergent-Assisted Glycoprotein Capture: A Versatile Tool for In-Depth N-Glycoproteome Analysis. J Proteome Res, 15(6):2080-6(2016) [pubmed]

10.1021/acs.jproteome.6b00056;

Chen R, Zou H, Figeys D, Detergent-Assisted Glycoprotein Capture: A Versatile Tool for In-Depth N-Glycoproteome Analysis. J Proteome Res, 15(6):2080-6(2016) [pubmed]

Keyword List

curator keyword: Technical
submitter keyword: LC-MS, human serum,N-glycoproteome, HEK 293

curator keyword: Technical

submitter keyword: LC-MS, human serum,N-glycoproteome, HEK 293

Contact List

Daniel Figeys
contact affiliation	Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology and Department of Chemistry and Biomolecular Sciences, University of Ottawa
contact email	dfigeys@uottawa.ca
lab head
Rui Chen
contact affiliation	University of Ottawa
contact email	rche3@uottawa.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003485
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003485

PRIDE project URI

Repository Record List

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