PXD003462

PXD003462 is an original dataset announced via ProteomeXchange.

Title	Quantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique
Description	We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total of eight iTRAQ 8-plex experiments were performed.
HostingRepository	PRIDE
AnnounceDate	2016-04-18
AnnouncementXML	Submission_2016-04-18_08:39:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guijie Zhu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	deamidated residue; monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-14 01:47:43	ID requested
⏵ 1	2016-04-18 08:39:18	announced

Zhu G, Sun L, Albanetti T, Linkous T, Larkin C, Schoner R, McGivney JB, Dovichi NJ, Quantitative analysis of the supernatant from host and transfected CHO cells using iTRAQ 8-plex technique. Biotechnol Bioeng, 113(10):2140-8(2016) [pubmed]

curator keyword: Biological

submitter keyword: CHO supernatant, iTRAQ 8-PLEX, LC-MS/MS

Norman Dovichi
contact affiliation	University of Notre Dame
contact email	ndovichi@nd.edu
lab head
Guijie Zhu
contact affiliation	University of Notre Dame
contact email	gzhu@nd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003462
     1. Label: PRIDE project
     2. Name: Quantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique