PXD003451

PXD003451 is an original dataset announced via ProteomeXchange.

Title	Reduced insulin/IGF-1 signaling restores the dynamic properties of key stress granule proteins during aging
Description	Low complexity “prion-like” domains in key RNA-binding proteins (RBPs) mediate the reversible assembly of RNA granules. Individual RBPs harboring these domains have been linked to specific neurodegenerative diseases. Although their aggregation in neurodegeneration has been extensively characterized, it remains unknown how the process of aging disturbs RBP dynamics. We show that a wide variety of RNA granule components including stress granule proteins become highly insoluble with age in C. elegans and that reduced insulin/IGF-1 daf-2 receptor signaling efficiently prevents their aggregation. Importantly, stress granule-related RBP aggregates are associated with reduced fitness. We show that HSF-1 is a main regulator of stress granule-related RBP aggregation in both young and aged animals. During aging, increasing DAF-16 activity restores dynamic stress granule-related RBPs partly by reducing the build-up of other misfolded proteins that seed RBP aggregation. Longevity-associated mechanisms found to maintain dynamic RBPs during aging could be relevant for neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:27:56.461.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Trinidad
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-12 01:49:49	ID requested
1	2017-01-16 14:20:57	announced
⏵ 2	2024-10-22 04:27:58	announced	2024-10-22: Updated project metadata.

Lechler MC, Crawford ED, Groh N, Widmaier K, Jung R, Kirstein J, Trinidad JC, Burlingame AL, David DC, Reduced Insulin/IGF-1 Signaling Restores the Dynamic Properties of Key Stress Granule Proteins during Aging. Cell Rep, 18(2):454-467(2017) [pubmed]

10.1016/j.celrep.2016.12.033;

curator keyword: Biomedical

submitter keyword: Caenorhabditis elegans, iTRAQ, quantification, aging, insulin, aggregation

Della C. David
contact affiliation	German Center for Neurodegenerative Diseases (DZNE), Tuebingen, Germany
contact email	dellacdavid@gmail.com
lab head
Jonathan Trinidad
contact affiliation	Indiana University
contact email	trinidad@indiana.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD003451

PRIDE project URI

• PRIDE
  1. PXD003451
     1. Label: PRIDE project
     2. Name: Reduced insulin/IGF-1 signaling restores the dynamic properties of key stress granule proteins during aging