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PXD003451

PXD003451 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReduced insulin/IGF-1 signaling restores the dynamic properties of key stress granule proteins during aging
DescriptionLow complexity “prion-like” domains in key RNA-binding proteins (RBPs) mediate the reversible assembly of RNA granules. Individual RBPs harboring these domains have been linked to specific neurodegenerative diseases. Although their aggregation in neurodegeneration has been extensively characterized, it remains unknown how the process of aging disturbs RBP dynamics. We show that a wide variety of RNA granule components including stress granule proteins become highly insoluble with age in C. elegans and that reduced insulin/IGF-1 daf-2 receptor signaling efficiently prevents their aggregation. Importantly, stress granule-related RBP aggregates are associated with reduced fitness. We show that HSF-1 is a main regulator of stress granule-related RBP aggregation in both young and aged animals. During aging, increasing DAF-16 activity restores dynamic stress granule-related RBPs partly by reducing the build-up of other misfolded proteins that seed RBP aggregation. Longevity-associated mechanisms found to maintain dynamic RBPs during aging could be relevant for neurodegenerative diseases.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:27:56.461.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonathan Trinidad
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListiTRAQ4plex-116 reporter+balance reagent acylated residue
InstrumentQSTAR
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-12 01:49:49ID requested
12017-01-16 14:20:57announced
22024-10-22 04:27:58announced2024-10-22: Updated project metadata.
Publication List
Lechler MC, Crawford ED, Groh N, Widmaier K, Jung R, Kirstein J, Trinidad JC, Burlingame AL, David DC, Reduced Insulin/IGF-1 Signaling Restores the Dynamic Properties of Key Stress Granule Proteins during Aging. Cell Rep, 18(2):454-467(2017) [pubmed]
10.1016/j.celrep.2016.12.033;
Keyword List
curator keyword: Biomedical
submitter keyword: Caenorhabditis elegans, iTRAQ, quantification, aging, insulin, aggregation
Contact List
Della C. David
contact affiliationGerman Center for Neurodegenerative Diseases (DZNE), Tuebingen, Germany
contact emaildellacdavid@gmail.com
lab head
Jonathan Trinidad
contact affiliationIndiana University
contact emailtrinidad@indiana.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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