PXD003450

PXD003450 is an original dataset announced via ProteomeXchange.

Title	Anopheles culicifacies species A salivary gland proteome
Description	Background: Anopheles culicifacies is a rural vector of malaria in tropical and sub tropical South East Asian region. The salivary gland of the mosquito is the target for sporozoite interaction, blood feeding behavior, haemostasis and vector-parasite interactions. Malaria parasite matures inside the salivary gland, gain competence and transmitted to the host along with the saliva during biting. The importance of the proteins expressed in salivary gland is the first step in understanding the physiology of blood feeding and may provide insights into vector- parasite interactions. Since, no genomic or transcriptomics information is available of Anopheles culicifacies, therefore locally expressed functional proteins in salivary glands are of much importance. . Method: In this study, 1DE protein and in solution digestion was combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for data mining was employed to study the proteome profile of salivary glands of sugar fed An. culicifacies mosquito species. Functional annotation of all the identified proteins was carried out using gene ontology tools, CELLO and SMART analysis software. Results: Total 102 proteins were identified and analysed by SEQUEST algorithm against mosquito protein database from Uniprot/NCBI. Out of which 81 proteins were identified using gel free approach and 21 proteins using in-gel approach and 15 were common among these two approaches. All the identified proteins were categorized in to 23 groups of biological processes using GO tool. 7 proteins were depicted to be secretary in nature by investigating the signal peptide present. Potential proteins with unknown function were predicted by analyzing their functional association with other characterized proteins by STRING algorithm and were categorized in cell adhesion, cytoskeleton and membrane trafficking networks. Conclusion: Our study elucidates the first proteomic dataset of An. culicifacies salivary gland proteins. Functional annotation of salivary proteins and complementary gene ontology assignments in An. culicifacies species may contribute towards understanding the complex physiology of the tissues in this species. This proteome baseline data may facilitate the discernment of salivary glands and parasite correlation during blood feeding. Furthermore, this mass spectrometry based proteomic data may also provide insights into the elucidation of role of differential functional proteins present in refractory An. culicifacies mosquito and may be useful for development of effective malaria control strategies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:25:55.494.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ritu Rawal
SpeciesList	scientific name: Anopheles culicifacies A; NCBI TaxID: 63366;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Thermo Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-12 01:27:57	ID requested
1	2016-09-13 03:29:55	announced
⏵ 2	2024-10-22 04:25:56	announced	2024-10-22: Updated project metadata.

10.1371/journal.pone.0161870;

Rawal R, Vijay S, Kadian K, Singh J, Pande V, Sharma A, Towards a Proteomic Catalogue and Differential Annotation of Salivary Gland Proteins in Blood Fed Malaria Vector Anopheles culicifacies by Mass Spectrometry. PLoS One, 11(9):e0161870(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: proteomics,Anopheles culicifacies, salivary gland, 1D electrophoresis, mass spectrometry, proteome

Dr. Arun Sharma
contact affiliation	*Dr. Arun Sharma Protein Biochemistry and Structural Biology Division National Institute of Malaria Research (ICMR) Department of Health Research, Ministry of Health and Family Welfare, Govt. of India Sector-8, Dwarka, New Delhi- 110077
contact email	arushar@gmail.com
lab head
Ritu Rawal
contact affiliation	Lab No. 125, Protein biochemistry lab, National Institute of Malaria Research, Dwarka , New Delhi-110077, India
contact email	ritu_1jan@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003450
     1. Label: PRIDE project
     2. Name: Anopheles culicifacies species A salivary gland proteome