PXD003449

PXD003449 is an original dataset announced via ProteomeXchange.

Title	Comparative Transcriptomics and Proteomics on Pi Depleted Arabidopsis Roots
Description	Plant adaptation to limited phosphate (Pi) availability comprises a wide range of strategies to conserve and remobilize internal Pi stores and to enhance Pi acquisition. Vigorous restructuring of root system architecture provides a developmental strategy for topsoil exploration and Pi scavenging. Changes in external Pi availability are locally sensed at the root tip and adjust root growth by modulating cell expansion and cell division. The functionally interacting Arabidopsis genes, LPR1/LPR2 and PDR2, are key components of root Pi sensing. A series of genome-wide studies revealed insight into transcriptional changes upon Pi starvation and provided a comprehensive overview of gene expression patterns in roots and photosynthetic tissues. In this study, we combined genome-wide transcriptome and proteome profiling in Pi-starved roots of wild-type, pdr2 and lpr1lpr2 seedlings. A comparative analysis of the datasets, combined with physiological and cell biological experiments, reveals a network regulating local root Fe uptake, storage and distribution upon Pi limitation. We further highlight expressional changes of several cell wall-modifying enzymes and provide evidence for a dynamic adjustment of the pectin network at sites of local Fe accumulation within the root meristem.
HostingRepository	PRIDE
AnnounceDate	2016-05-06
AnnouncementXML	Submission_2016-05-06_04:31:13.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003449
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wolfgang Hoehenwarter
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-12 00:19:00	ID requested
⏵ 1	2016-05-06 04:31:15	announced

Hoehenwarter W, M, ö, nchgesang S, Neumann S, Majovsky P, Abel S, M, ü, ller J, Comparative expression profiling reveals a role of the root apoplast in local phosphate response. BMC Plant Biol, 16():106(2016) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, Root, Phosphate, Proteomics

Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
lab head
Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003449
     1. Label: PRIDE project
     2. Name: Comparative Transcriptomics and Proteomics on Pi Depleted Arabidopsis Roots