PXD003445

PXD003445 is an original dataset announced via ProteomeXchange.

Title	In vitro chromatin assembly - A quantitative proteomic analysis of in vitro assembled chromatin
Description	Here, we use LC-MSMS and SWATH-MS to describe the kinetics of in vitro assembled chromatin supported by an embryo extract prepared from preblastoderm Drosophila melanogaster embryos. This system allows easy manipulation of distinct aspects of chromatin assembly such as post-translational histone modifications, the levels of histone chaperones and the concentration of distinct DNA binding factors. Our findings support the idea that chromatin assembly factors and factors important for chromatin structure bind chromatin an ordered manner, which is -at least in part- regulated by histone deacetylation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:00.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Voelker-Albert
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-11 06:28:57	ID requested
1	2016-01-27 07:42:31	announced
⏵ 2	2024-10-22 04:28:04	announced	2024-10-22: Updated project metadata.

V, ö, lker-Albert MC, Pusch MC, Fedisch A, Schilcher P, Schmidt A, Imhof A, A Quantitative Proteomic Analysis of In Vitro Assembled Chromatin. Mol Cell Proteomics, 15(3):945-59(2016) [pubmed]

curator keyword: Technical, Biological

submitter keyword: LC-MSMS, Chromatin Assembly,Drosophila melanogaster

Axel Imhof
contact affiliation	Histone Modifications Group Zentrallabor für Proteinanalytik BioMedical Center Ludwig-Maximilians-University of Munich Großhadernerstr. 9 82152 Planegg-Martinsried GERMANY
contact email	imhof@lmu.de
lab head
Moritz Voelker-Albert
contact affiliation	LMU Munich
contact email	Moritz.Voelker-Albert@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003445

PRIDE project URI

• PRIDE
  1. PXD003445
     1. Label: PRIDE project
     2. Name: In vitro chromatin assembly - A quantitative proteomic analysis of in vitro assembled chromatin