PXD003444

PXD003444 is an original dataset announced via ProteomeXchange.

Title	The Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis
Description	Porphyrmonas gingivalis is an oral pathogen associated with the inflammatory disease periodontitis. The colonization of oral epithelial surfaces by P. gingivalis may also lead to the autoimmune disease rheumatoid arthritis. One of the hallmarks of rheumatoid arthritis is the loss of tolerance against citrullinated proteins. Citrullination is a post-translational modification of arginine residues, leading to a change in structure and function of the respective protein. This modification is catalysed by peptidylarginine deiminases (PAD). Interestingly, P. gingivalis secretes a citrullinating enzyme, known as P. gingivalis PAD (PPAD), which targets bacterial and human proteins. Since the extent of P. gingivalis protein citrullination by PPAD was not yet known, the present study was aimed at identifying the extracellular proteome and citrullinome of P. gingivalis. To this end, extracellular proteins of two reference strains, two PPAD-deficient mutants and three clinical isolates of P. gingivalis were analysed by mass spectrometry. The results uncovered substantial heterogeneity in the extracellular proteome and citrullinome of P. gingivalis, especially in relation to the extracellular detection of typical cytoplasmic proteins. In contrast, major virulence factors were identified in all investigated isolates although their citrullination was shown to vary. This may be related to post-translational processing of the PPAD enzyme.
HostingRepository	PRIDE
AnnounceDate	2018-02-28
AnnouncementXML	Submission_2019-02-12_07:57:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Otto
SpeciesList	scientific name: Porphyromonas gingivalis; NCBI TaxID: 837;
ModificationList	L-citrulline
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-11 04:35:13	ID requested
1	2018-02-28 05:23:37	announced
⏵ 2	2019-02-12 07:57:05	announced	Updated publication reference for PubMed record(s): 27712078.

Stobernack T, Glasner C, Junker S, Gabarrini G, de Smit M, de Jong A, Otto A, Becher D, van Winkelhoff AJ, van Dijl JM, Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis. J Proteome Res, 15(12):4532-4543(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Porphyromonas gingivalis, exoproteome, citrullination, mass spectrometry, periodontitis, rheumatoid arthritis

Andreas Otto
contact affiliation	Institute for Microbiology Department of Microbial Proteomics and Mass Spectrometry Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 15 17489 Greifswald phone: ++49 3834 864073 ++49 3834 864072
contact email	andreas.otto@uni-greifswald.de
lab head
Andreas Otto
contact affiliation	Institute for Microbiology
contact email	andreas.otto@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD003444

PRIDE project URI

• PRIDE
  1. PXD003444
     1. Label: PRIDE project
     2. Name: The Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis