PXD003439 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome profiling of Bacillus subtilis stressed with the antibiotics tetracycline and chelocardin |
Description | Chelocardin is an atypical tetracycline and its mechanism of action is discussed controversially in literature. Therefore, we analyzed the bacterial response of Bacillus subtilis to chelocardin and as a control for classical tetracyclines to tetracycline by proteomic profiling. The induced marker proteins mirror the damage in the cell due the antibiotic stress. Proteomic profiling was carried out by 2D-PAGE and spot identification by nUPLC-ESI-MS/MS. The proteomic profile of chelocardin demonstrates a dual mechanism of action with protein biosynthesis inhibition and membrane damage. |
HostingRepository | PRIDE |
AnnounceDate | 2016-03-14 |
AnnouncementXML | Submission_2016-03-14_06:05:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jennifer Stepanek |
SpeciesList | scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308; |
ModificationList | deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-01-11 01:12:09 | ID requested | |
1 | 2016-03-14 04:41:14 | announced | |
⏵ 2 | 2016-03-14 06:05:43 | announced | Updated publication reference for PubMed record(s): 26969785. |
Publication List
Stepanek JJ, Luke, ž, i, č T, Teichert I, Petkovi, ć H, Bandow JE, Dual mechanism of action of the atypical tetracycline chelocardin. Biochim Biophys Acta, 1864(6):645-654(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Bacillus subtilis tetracycline chelocardin 2D-PAGE Proteomics |
Contact List
Julia Elisabeth Bandow |
contact affiliation | Ruhr-Universität Bochum, AG Angewandte Mikrobiologie, Universitätsstraße 150, 44780 Bochum, Raum: ND 06/795, Tel.: +49 234 32 23102 |
contact email | julia.bandow@rub.de |
lab head | |
Jennifer Stepanek |
contact affiliation | Ruhr-University Bochum |
contact email | Jennifer.stepanek@rub.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD003439 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003439
- Label: PRIDE project
- Name: Proteome profiling of Bacillus subtilis stressed with the antibiotics tetracycline and chelocardin