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PXD003439

PXD003439 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome profiling of Bacillus subtilis stressed with the antibiotics tetracycline and chelocardin
DescriptionChelocardin is an atypical tetracycline and its mechanism of action is discussed controversially in literature. Therefore, we analyzed the bacterial response of Bacillus subtilis to chelocardin and as a control for classical tetracyclines to tetracycline by proteomic profiling. The induced marker proteins mirror the damage in the cell due the antibiotic stress. Proteomic profiling was carried out by 2D-PAGE and spot identification by nUPLC-ESI-MS/MS. The proteomic profile of chelocardin demonstrates a dual mechanism of action with protein biosynthesis inhibition and membrane damage.
HostingRepositoryPRIDE
AnnounceDate2016-03-14
AnnouncementXMLSubmission_2016-03-14_06:05:42.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJennifer Stepanek
SpeciesList scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationListdeamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-11 01:12:09ID requested
12016-03-14 04:41:14announced
22016-03-14 06:05:43announcedUpdated publication reference for PubMed record(s): 26969785.
Publication List
Stepanek JJ, Luke, ž, i, č T, Teichert I, Petkovi, ć H, Bandow JE, Dual mechanism of action of the atypical tetracycline chelocardin. Biochim Biophys Acta, 1864(6):645-654(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Bacillus subtilis tetracycline chelocardin 2D-PAGE Proteomics
Contact List
Julia Elisabeth Bandow
contact affiliationRuhr-Universität Bochum, AG Angewandte Mikrobiologie, Universitätsstraße 150, 44780 Bochum, Raum: ND 06/795, Tel.: +49 234 32 23102
contact emailjulia.bandow@rub.de
lab head
Jennifer Stepanek
contact affiliationRuhr-University Bochum
contact emailJennifer.stepanek@rub.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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