PXD003438

PXD003438 is an original dataset announced via ProteomeXchange.

Title	SCX_MS raw files and RP_MS raw files
Description	A novel one-dimensional on-line pH gradient-eluted strong cation exchange (SCX)-nano-ESI-MS/MS method was developed for protein identification and tested with mixture of six standard proteins, total lysate of HuH7 and N2a cells, as well as membrane fraction of N2a cells. This method utilized an on-line nano-flow SCX column in a nano-LC system coupled with a nano-electrospray high-resolution mass spectrometer. Protein digests were separated on a nano-flow SCX column with a pH gradient and directly introduced into a mass spectrometer through nano-electrospray ionization. SCXLC-MS/MS showed identification capability for higher proportion of basic peptides compared to RPLC-MS/MS method, especially for histidine-containing peptides. Our SCXLC-MS/MS method is an excellent alternative method to the RPLC-MS/MS method for analysis of standard proteins, total cell and membrane proteomes.
HostingRepository	PRIDE
AnnounceDate	2016-01-28
AnnouncementXML	Submission_2016-01-28_06:48:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	mai dier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-11 00:48:51	ID requested
⏵ 1	2016-01-28 06:48:50	announced

Xu J, Gao J, Yu C, He H, Yang Y, Figeys D, Zhou H, Development of Online pH Gradient-Eluted Strong Cation Exchange Nanoelectrospray-Tandem Mass Spectrometry for Proteomic Analysis Facilitating Basic and Histidine-Containing Peptides Identification. Anal Chem, 88(1):583-91(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: pH gradient, strong cation exchange

Hu Zhou
contact affiliation	Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China
contact email	zhouhu@simm.ac.cn
lab head
mai dier
contact affiliation	SIMM
contact email	925722431@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003438

PRIDE project URI

• PRIDE
  1. PXD003438
     1. Label: PRIDE project
     2. Name: SCX_MS raw files and RP_MS raw files