PXD003435
PXD003435 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Synthetic yeast genome project |
Description | The experiment aimed to study the proteome variation in a yeast strain (YS031), which chromosome II was artificially synthezied, compared with the wild type strain (BY4741). Proteins were extracted from the yeast cells and digested with trypsin. The peptides were label with iTRAQ, separated with 2D LC (SCX and RP) and detected with a Q-Exactive MS. Proteome identification and quantification were performed with Mascot2.3 and iQuant3.0. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-27 |
AnnouncementXML | Submission_2018-10-27_03:37:00.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003435 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Baojin Zhou |
SpeciesList | scientific name: Saccharomyces cerevisiae (baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide -site C: 57.021464; monohydroxylated residue: 15.994915; iTRAQ8plex-121 reporter fragment; iodoacetamide - site C: 57.021464 |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2016-01-08 01:17:00 | ID requested | |
⏵ 1 | 2018-10-27 03:37:01 | announced |
Publication List
Shen Y, Wang Y, Chen T, Gao F, Gong J, Abramczyk D, Walker R, Zhao H, Chen S, Liu W, Luo Y, M, ΓΌ, ller CA, Paul-Dubois-Taine A, Alver B, Stracquadanio G, Mitchell LA, Luo Z, Fan Y, Zhou B, Wen B, Tan F, Wang Y, Zi J, Xie Z, Li B, Yang K, Richardson SM, Jiang H, French CE, Nieduszynski CA, Koszul R, Marston AL, Yuan Y, Wang J, Bader JS, Dai J, Boeke JD, Xu X, Cai Y, Yang H, Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome. Science, 355(6329):(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: iTRAQ, LC-MS/MS, Saccharomyces cerevisiae |
Contact List
Bo Wen | |
---|---|
contact affiliation | BGI-Shenzhen |
contact email | wenbo@genomics.cn |
lab head | |
Baojin Zhou | |
contact affiliation | BGI-Shenzhen |
contact email | zhoubaojin@genomics.cn |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003435 |
PRIDE project URI |
Repository Record List
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