PXD003435

PXD003435 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Synthetic yeast genome project
Description	The experiment aimed to study the proteome variation in a yeast strain (YS031), which chromosome II was artificially synthezied, compared with the wild type strain (BY4741). Proteins were extracted from the yeast cells and digested with trypsin. The peptides were label with iTRAQ, separated with 2D LC (SCX and RP) and detected with a Q-Exactive MS. Proteome identification and quantification were performed with Mascot2.3 and iQuant3.0.
HostingRepository	PRIDE
AnnounceDate	2018-10-27
AnnouncementXML	Submission_2018-10-27_03:37:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003435
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Baojin Zhou
SpeciesList	scientific name: Saccharomyces cerevisiae (baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide -site C: 57.021464; monohydroxylated residue: 15.994915; iTRAQ8plex-121 reporter fragment; iodoacetamide - site C: 57.021464
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-08 01:17:00	ID requested
⏵ 1	2018-10-27 03:37:01	announced

Publication List

Shen Y, Wang Y, Chen T, Gao F, Gong J, Abramczyk D, Walker R, Zhao H, Chen S, Liu W, Luo Y, M, ü, ller CA, Paul-Dubois-Taine A, Alver B, Stracquadanio G, Mitchell LA, Luo Z, Fan Y, Zhou B, Wen B, Tan F, Wang Y, Zi J, Xie Z, Li B, Yang K, Richardson SM, Jiang H, French CE, Nieduszynski CA, Koszul R, Marston AL, Yuan Y, Wang J, Bader JS, Dai J, Boeke JD, Xu X, Cai Y, Yang H, Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome. Science, 355(6329):(2017) [pubmed]

Shen Y, Wang Y, Chen T, Gao F, Gong J, Abramczyk D, Walker R, Zhao H, Chen S, Liu W, Luo Y, M, ü, ller CA, Paul-Dubois-Taine A, Alver B, Stracquadanio G, Mitchell LA, Luo Z, Fan Y, Zhou B, Wen B, Tan F, Wang Y, Zi J, Xie Z, Li B, Yang K, Richardson SM, Jiang H, French CE, Nieduszynski CA, Koszul R, Marston AL, Yuan Y, Wang J, Bader JS, Dai J, Boeke JD, Xu X, Cai Y, Yang H, Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome. Science, 355(6329):(2017) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: iTRAQ, LC-MS/MS, Saccharomyces cerevisiae

curator keyword: Biological

submitter keyword: iTRAQ, LC-MS/MS, Saccharomyces cerevisiae

Contact List

Bo Wen
contact affiliation	BGI-Shenzhen
contact email	wenbo@genomics.cn
lab head
Baojin Zhou
contact affiliation	BGI-Shenzhen
contact email	zhoubaojin@genomics.cn
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003435
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003435

PRIDE project URI

Repository Record List

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