PXD003434

PXD003434 is an original dataset announced via ProteomeXchange.

Title	Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization
Description	Tissue samples from human tonsils and rat pancreas were subjected to a novel laser-based homogenization method, the PIRL-DIVE homogenization, and to a conventional mechanical tissue homogenization procedure (bead mill and a cryo-grinder). The protein species composition of the protein extracts were compared by differential proteome analysis using two-dimensional gel electrophoresis followed by LC-MS/MS analysis and one-dimensional gel electrophoresis (SDS-PAGE) followed by quantitative LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2016-01-25
AnnouncementXML	Submission_2016-01-25_10:52:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcus Wurlitzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-08 00:10:31	ID requested
⏵ 1	2016-01-25 10:52:49	announced

Kwiatkowski M, Wurlitzer M, Krutilin A, Kiani P, Nimer R, Omidi M, Mannaa A, Bussmann T, Bartkowiak K, Kruber S, Uschold S, Steffen P, L, ü, bberstedt J, K, ü, pker N, Petersen H, Knecht R, Hansen NO, Zarrine-Afsar A, Robertson WD, Miller RJD, Schl, ü, ter H, Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization. J Proteomics, 134():193-202(2016) [pubmed]

curator keyword: Technical

submitter keyword: LC-MSMS, sample preparation, tissue homogenization, protein species, proteolysis, PIRL, PIRL-DIVE

Hartmut Schlüter
contact affiliation	Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	hschluet@uke.de
lab head
Marcus Wurlitzer
contact affiliation	Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	m.wurlitzer@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003434

PRIDE project URI

• PRIDE
  1. PXD003434
     1. Label: PRIDE project
     2. Name: Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization