PXD003429

PXD003429 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison of uninfected and wMelPop infected Aag2 cells
Description	Wolbachia endosymbionts are widespread intracellular, maternally inherited bacteria which manipulate the host to favour their spread through a population. Cytoplasmic incompatibility (CI) and viral suppression are two such manipulations driven by uncharacterised mechanisms. To gain insight into potential molecular mechanisms responsible for these effects, we performed the first in-depth proteomic characterisation of the host response to Wolbachia infection. We find that the presence of the Wolbachia wMelPop in Aedes aegypti mosquito cells alters levels of proteins involved in cell cycle control, DNA replication, autophagy, vesicular trafficking, iron homeostasis, amino acid degradation, purine metabolism, lipid metabolism and immunity. The majority of the cell cycle/DNA replication proteins were downregulated in the presence of Wolbachia, in particular, a member of the anoctamin family was strongly down regulated which may be related to the chromosomal segregation defects observed in CI crosses. We found clear evidence for perturbed lipid metabolism, Wolbachia infected cells expressed higher levels of Apolipoprotein D and the cholesterol efflux transporter ABCA1, while the LDL receptor and fatty acid synthase were downregulated. ABCA1 was also upregulated at the mRNA level in adult Wolbachia infected Aedes aegypti mosquitoes. Wolbachia therefore perturbs cholesterol homeostasis causing the host cell to respond to an apparent cholesterol excess which may contribute to viral inhibition.
HostingRepository	PRIDE
AnnounceDate	2017-09-19
AnnouncementXML	Submission_2017-09-19_04:38:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vincent Geoghegan
SpeciesList	scientific name: Aedes aegypti (Yellowfever mosquito) (Culex aegypti); NCBI TaxID: 7159;
ModificationList	2x(13)C: 4x(2)H labeled dimethylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-07 01:49:50	ID requested
⏵ 1	2017-09-19 04:38:04	announced

Geoghegan V, Stainton K, Rainey SM, Ant TH, Dowle AA, Larson T, Hester S, Charles PD, Thomas B, Sinkins SP, Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells. Nat Commun, 8(1):526(2017) [pubmed]

curator keyword: Biological

submitter keyword: Aag2, wolbachia, dimethyl, offgel, wMelPop, aedes aegypti, mass spectrometry, cholesterol, cytoplasmic incompatibility, wAlbB

Steven P. Sinkins
contact affiliation	Biological and Life Sciences, Lancaster University
contact email	s.sinkins@lancaster.ac.uk
lab head
Vincent Geoghegan
contact affiliation	Lancaster University
contact email	v.geoghegan1@lancs.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003429
     1. Label: PRIDE project
     2. Name: Proteomic comparison of uninfected and wMelPop infected Aag2 cells