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PXD003423

PXD003423 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells
DescriptionThe goal of the present project was to investigate the EGF-dependent ubiquitination changes occurring in HeLa cells upon a CYLD deficiency. We applied SILAC-based quantitative mass spectrometry proteomics together with genetic engineering and biochemical approaches, in order to enrich the ubiquitinated proteins of the control and silenced cells in response to the growth factor. In order to get a deeper insight of the obtained results, we subsequently combined the enrichment of CYLD protein through immunoprecipitation with SILAC-based proteomics. This allowed us to decipher a specific tyrosine phosphorylation of CYLD that responds to the treatment with EGF and, therefore, we next aimed to analyse the ability of this modification to recruit potential interaction partners. We thus performed a peptide pull-down assay that uncovered a set of proteins specifically associated to the phosphorylated tyrosine and helped to uncover an unexpected, previously unreported role for this deubiquitinase.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:37:08.614.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVIRGINIA Sanchez-Quiles
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-06 00:05:14ID requested
12017-06-14 04:19:47announced
22024-10-22 04:37:12announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.M116.066423;
Sanchez-Quiles V, Akimov V, Osinalde N, Francavilla C, Puglia M, Barrio-Hernandez I, Kratchmarova I, Olsen JV, Blagoev B, Cylindromatosis Tumor Suppressor Protein (CYLD) Deubiquitinase is Necessary for Proper Ubiquitination and Degradation of the Epidermal Growth Factor Receptor. Mol Cell Proteomics, 16(8):1433-1446(2017) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Ubiquitination, EGF, CYLD
Contact List
BLAGOY BLAGOEV
contact affiliationUNIVERSITY OF SOUTHERN DENMARK
contact emailbab@bmb.sdu.dk
lab head
VIRGINIA Sanchez-Quiles
contact affiliationCurie Institute
contact emailvirginia.sanchez-quiles@curie.fr
dataset submitter
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Dataset FTP location
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