PXD003423 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells |
Description | The goal of the present project was to investigate the EGF-dependent ubiquitination changes occurring in HeLa cells upon a CYLD deficiency. We applied SILAC-based quantitative mass spectrometry proteomics together with genetic engineering and biochemical approaches, in order to enrich the ubiquitinated proteins of the control and silenced cells in response to the growth factor. In order to get a deeper insight of the obtained results, we subsequently combined the enrichment of CYLD protein through immunoprecipitation with SILAC-based proteomics. This allowed us to decipher a specific tyrosine phosphorylation of CYLD that responds to the treatment with EGF and, therefore, we next aimed to analyse the ability of this modification to recruit potential interaction partners. We thus performed a peptide pull-down assay that uncovered a set of proteins specifically associated to the phosphorylated tyrosine and helped to uncover an unexpected, previously unreported role for this deubiquitinase. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:37:08.614.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | VIRGINIA Sanchez-Quiles |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-01-06 00:05:14 | ID requested | |
1 | 2017-06-14 04:19:47 | announced | |
⏵ 2 | 2024-10-22 04:37:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.M116.066423; |
Sanchez-Quiles V, Akimov V, Osinalde N, Francavilla C, Puglia M, Barrio-Hernandez I, Kratchmarova I, Olsen JV, Blagoev B, Cylindromatosis Tumor Suppressor Protein (CYLD) Deubiquitinase is Necessary for Proper Ubiquitination and Degradation of the Epidermal Growth Factor Receptor. Mol Cell Proteomics, 16(8):1433-1446(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Ubiquitination, EGF, CYLD |
Contact List
BLAGOY BLAGOEV |
contact affiliation | UNIVERSITY OF SOUTHERN DENMARK |
contact email | bab@bmb.sdu.dk |
lab head | |
VIRGINIA Sanchez-Quiles |
contact affiliation | Curie Institute |
contact email | virginia.sanchez-quiles@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003423
- Label: PRIDE project
- Name: EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells