PXD003423

PXD003423 is an original dataset announced via ProteomeXchange.

Title	EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells
Description	The goal of the present project was to investigate the EGF-dependent ubiquitination changes occurring in HeLa cells upon a CYLD deficiency. We applied SILAC-based quantitative mass spectrometry proteomics together with genetic engineering and biochemical approaches, in order to enrich the ubiquitinated proteins of the control and silenced cells in response to the growth factor. In order to get a deeper insight of the obtained results, we subsequently combined the enrichment of CYLD protein through immunoprecipitation with SILAC-based proteomics. This allowed us to decipher a specific tyrosine phosphorylation of CYLD that responds to the treatment with EGF and, therefore, we next aimed to analyse the ability of this modification to recruit potential interaction partners. We thus performed a peptide pull-down assay that uncovered a set of proteins specifically associated to the phosphorylated tyrosine and helped to uncover an unexpected, previously unreported role for this deubiquitinase.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:08.614.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VIRGINIA Sanchez-Quiles
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-06 00:05:14	ID requested
1	2017-06-14 04:19:47	announced
⏵ 2	2024-10-22 04:37:12	announced	2024-10-22: Updated project metadata.

10.1074/mcp.M116.066423;

Sanchez-Quiles V, Akimov V, Osinalde N, Francavilla C, Puglia M, Barrio-Hernandez I, Kratchmarova I, Olsen JV, Blagoev B, Cylindromatosis Tumor Suppressor Protein (CYLD) Deubiquitinase is Necessary for Proper Ubiquitination and Degradation of the Epidermal Growth Factor Receptor. Mol Cell Proteomics, 16(8):1433-1446(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Ubiquitination, EGF, CYLD

BLAGOY BLAGOEV
contact affiliation	UNIVERSITY OF SOUTHERN DENMARK
contact email	bab@bmb.sdu.dk
lab head
VIRGINIA Sanchez-Quiles
contact affiliation	Curie Institute
contact email	virginia.sanchez-quiles@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD003423

PRIDE project URI

• PRIDE
  1. PXD003423
     1. Label: PRIDE project
     2. Name: EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells