PXD003411

PXD003411 is an original dataset announced via ProteomeXchange.

Title	Contribution of primary human fibroblasts and endothelial cells to the hallmarks of inflammation as determined by proteome profiling - secreted proteins of inflammatory stimulated endothelial cells
Description	While the most important players of inflammation have been well described, a systematic analysis of the proteins fulfilling the effector functionalities during inflammation has not yet been undertaken. Here we present a systematic proteome study of inflammatory activated primary human endothelial cells and fibroblasts. Cells were stimulated with interleukin 1-beta and fractionated in order to obtain secreted, cytoplasmic and nuclear protein fractions. Proteins were submitted to a data-dependent bottom up analytical platform using a QExactive orbitrap and the MaxQuant software for protein identification and label-free quantification. Results were further combined with similarly generated data previously obtained from the analysis of inflammatory activated peripheral blood mononuclear cells. Applying an FDR of less than 0.01 at both peptide and protein level, a total of 8235 protein groups assembled from 163858 peptides was identified. Comparative proteome analysis allowed us to determine proteins regulated in each kind of cells during inflammation. Remarkably, cells were working on similar inflammation-related tasks, however, by regulating different proteins. Thus, we were able to determine cell type-specific inflammatory signatures, apparently resulting from cell type-specific regulatory mechanisms. Hallmarks of inflammation emerged from these findings, representing commonly and cell type-specific responsibilities of cells during inflammation.
HostingRepository	PRIDE
AnnounceDate	2016-04-04
AnnouncementXML	Submission_2016-04-04_04:35:09.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003411
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-05 02:29:10	ID requested
⏵ 1	2016-04-04 04:35:12	announced

Slany A, Bileck A, Kreutz D, Mayer RL, Muqaku B, Gerner C, Contribution of Human Fibroblasts and Endothelial Cells to the Hallmarks of Inflammation as Determined by Proteome Profiling. Mol Cell Proteomics, 15(6):1982-97(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Cell type-specific inflammatory response, hallmarks of inflammation, mass spectrometry, primary human fibroblasts and endothelial cells, proteomics, secretome

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003411
     1. Label: PRIDE project
     2. Name: Contribution of primary human fibroblasts and endothelial cells to the hallmarks of inflammation as determined by proteome profiling - secreted proteins of inflammatory stimulated endothelial cells