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PXD003408

PXD003408 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleContribution of primary human fibroblasts and endothelial cells to the hallmarks of inflammation as determined by proteome profiling - nuclear proteins of untreated endothelial cells
DescriptionWhile the most important players of inflammation have been well described, a systematic analysis of the proteins fulfilling the effector functionalities during inflammation has not yet been undertaken. Here we present a systematic proteome study of inflammatory activated primary human endothelial cells and fibroblasts. Cells were stimulated with interleukin 1-beta and fractionated in order to obtain secreted, cytoplasmic and nuclear protein fractions. Proteins were submitted to a data-dependent bottom up analytical platform using a QExactive orbitrap and the MaxQuant software for protein identification and label-free quantification. Results were further combined with similarly generated data previously obtained from the analysis of inflammatory activated peripheral blood mononuclear cells. Applying an FDR of less than 0.01 at both peptide and protein level, a total of 8235 protein groups assembled from 163858 peptides was identified. Comparative proteome analysis allowed us to determine proteins regulated in each kind of cells during inflammation. Remarkably, cells were working on similar inflammation-related tasks, however, by regulating different proteins. Thus, we were able to determine cell type-specific inflammatory signatures, apparently resulting from cell type-specific regulatory mechanisms. Hallmarks of inflammation emerged from these findings, representing commonly and cell type-specific responsibilities of cells during inflammation.
HostingRepositoryPRIDE
AnnounceDate2016-04-04
AnnouncementXMLSubmission_2016-04-04_03:42:46.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003408
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Gerner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListL-histidine residue; residues isobaric at 128.058578 Da; Oxidation; L-tyrosine residue; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-05 02:26:39ID requested
12016-04-04 03:42:49announced
Publication List
Slany A, Bileck A, Kreutz D, Mayer RL, Muqaku B, Gerner C, Contribution of Human Fibroblasts and Endothelial Cells to the Hallmarks of Inflammation as Determined by Proteome Profiling. Mol Cell Proteomics, 15(6):1982-97(2016) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Cell type-specific inflammatory response, hallmarks of inflammation, mass spectrometry, primary human fibroblasts and endothelial cells, proteomics, secretome
Contact List
Christopher Gerner
contact affiliationUniversity of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact emailchristopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliationUniversity of Vienna
contact emailchristopher.gerner@univie.ac.at
dataset submitter
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Dataset FTP location
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